Increased Tau Phosphorylation and Tau Truncation, and Decreased Synaptophysin Levels in Mutant BRI2/Tau Transgenic Mice

Holly Garringer, Jill Murrell, Neeraja Sammeta, Anita Gnezda, Bernardino Ghetti, Ruben Vidal

Research output: Contribution to journalArticle

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Abstract

Familial Danish dementia (FDD) is an autosomal dominant neurodegenerative disease caused by a 10-nucleotide duplication-insertion in the BRI2 gene. FDD is clinically characterized by loss of vision, hearing impairment, cerebellar ataxia and dementia. The main neuropathologic findings in FDD are the deposition of Danish amyloid (ADan) and the presence of neurofibrillary tangles (NFTs). Here we investigated tau accumulation and truncation in double transgenic (Tg-FDD-Tau) mice generated by crossing transgenic mice expressing human Danish mutant BRI2 (Tg-FDD) with mice expressing human 4-repeat mutant Tau-P301S (Tg-Tau). Compared to Tg-Tau mice, we observed a significant enhancement of tau deposition in Tg-FDD-Tau mice. In addition, a significant increase in tau cleaved at aspartic acid (Asp) 421 was observed in Tg-FDD-Tau mice. Tg-FDD-Tau mice also showed a significant decrease in synaptophysin levels, occurring before widespread deposition of fibrillar ADan and tau can be observed. Thus, the presence of soluble ADan/mutant BRI2 can lead to significant changes in tau metabolism and synaptic dysfunction. Our data provide new in vivo insights into the pathogenesis of FDD and the pathogenic pathway(s) by which amyloidogenic peptides, regardless of their primary amino acid sequence, can cause neurodegeneration.

Original languageEnglish
Article numbere56426
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 13 2013

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Synaptophysin
Phosphorylation
dementia
Transgenic Mice
phosphorylation
genetically modified organisms
mutants
mice
Neurodegenerative diseases
Audition
Amyloid
Metabolism
Aspartic Acid
Nucleotides
Genes
Amino Acids
Peptides
Familial Danish dementia
Cerebellar Ataxia
Neurofibrillary Tangles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Increased Tau Phosphorylation and Tau Truncation, and Decreased Synaptophysin Levels in Mutant BRI2/Tau Transgenic Mice. / Garringer, Holly; Murrell, Jill; Sammeta, Neeraja; Gnezda, Anita; Ghetti, Bernardino; Vidal, Ruben.

In: PLoS One, Vol. 8, No. 2, e56426, 13.02.2013.

Research output: Contribution to journalArticle

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