Increased thymidylate synthase (EC 2.1.1.45) activity in normal and neoplastic proliferation.

Y. Hashimoto, T. Shiotani, John Eble, J. L. Glover, G. Weber

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Abstract

Thymidylate (dTMP) synthase (EC 2.1.1.45) activity was measured in 100,000 x g supernatant fluid with a sensitive, rapid radio assay. The activity in normal rat liver was low (0.098-0.204 nmol/hr/mg protein). dTMP synthase specific activities in rat thymus, spleen, bone marrow, testis, lung, heart, brain, kidney, and small intestine were 6297, 1842, 1500, 788, 215, 76, 61, 39 and 24%, respectively, of that of the liver. The activity in 5-day-old rat liver was 16-fold higher than in adult. dTMP synthase activity increased in rat hepatomas to 7- to 125-fold of that of normal rat liver. There was a significant correlation between the increase in synthase activity and the proliferation rates of the hepatomas. In 8 human colon carcinomas, dTMP synthase activity increased to 2.9- to 8-fold of that of normal human colon mucosa. In leukemic leukocytes from 3 leukemia patients, activity was 8- to 10-fold higher than in normal leukocytes.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCancer Biochemistry Biophysics
Volume10
Issue number1
StatePublished - Jul 1988

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Thymidylate Synthase
Liver
Hepatocellular Carcinoma
Colon
Leukocytes
Radio
Thymus Gland
Small Intestine
Testis
Leukemia
Mucous Membrane
Spleen
Bone Marrow
Carcinoma
Kidney
Lung
Brain
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Cancer Research

Cite this

Increased thymidylate synthase (EC 2.1.1.45) activity in normal and neoplastic proliferation. / Hashimoto, Y.; Shiotani, T.; Eble, John; Glover, J. L.; Weber, G.

In: Cancer Biochemistry Biophysics, Vol. 10, No. 1, 07.1988, p. 1-10.

Research output: Contribution to journalArticle

Hashimoto, Y. ; Shiotani, T. ; Eble, John ; Glover, J. L. ; Weber, G. / Increased thymidylate synthase (EC 2.1.1.45) activity in normal and neoplastic proliferation. In: Cancer Biochemistry Biophysics. 1988 ; Vol. 10, No. 1. pp. 1-10.
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