Increased transforming growth factor-ßl expression in regenerating rat renal tubules following ischémie injury

David P. Basile, Jason M. Rovak, Daniel R. Martin, Marc R. Hammerman

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

To gain insight into the role that transforming growth factor-ßl (TGF-ßl) plays in the regeneration of kidneys following acute renal failure, we characterized the expression of TGF-ßl mRNA and the expression of active and latent TGF-ß peptide at various times during recovery from acute ischémie injury in rat. Levels of whole kidney TGF-ßl mRNA were elevated significantly at 12 h postinjury (1.5-fold vs. sham-operated controls), and by 24 h postinjury were elevated by 3.6-fold. Levels remained elevated for 14 days following ischemia, but were no longer elevated at 28 days postinjury. In situ hybridization demonstrated that the elevated expression of TGF-ßl was localized predominantly to cells in the regenerating tubules in the outer medulla. When examined at 14 days postischemia, levels of TGF-ßl mRNA were elevated in the outer medulla only in tubules that appeared incompletely regenerated. Immunohistochemical staining localized active TGF-ß to the lumen of proximal tubules in control animals and in desquamated and regenerating tubular epithelial cells following ischemia. TGF-ßl latency-associated peptide was present intracellularly in proximal tubules of sham-operated rats and reduced following ischemia. We hypothesize that endogenous renal TGF-ß serves to promote tissue regeneration following acute injury via an autocrine or paracrine mechanism.

Original languageEnglish (US)
Pages (from-to)F500-F509
JournalAmerican Journal of Physiology
Volume270
Issue number3 PART 2
StatePublished - 1996

Keywords

  • Acute renal failure
  • Differentiation
  • Proximal tubule
  • Regeneration

ASJC Scopus subject areas

  • Physiology (medical)

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