Independent regulation of periarteriolar and perivenular nitric oxide mechanisms in the in vivo hamster cheek pouch microvasculature

David D. Kim, Takehito Kanetaka, Ricardo G. Durán, Fabiola A. Sánchez, H. Bohlen, Walter N. Durán

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: We tested the hypothesis that differential stimulation of nitric oxide (NO) production can be induced in pre- and postcapillary segments of the microcirculation in the hamster cheek pouch. Materials and Methods: We applied acetylcholine (ACh) or platelet-activating factor (PAF) topically and measured perivascular NO concentration ([NO]) with NO-sensitive microelectrodes in arterioles and venules of the hamster cheek pouch. We also measured NO in cultured coronary endothelial cells (CVEC) after ACh or PAF. Results: ACh increased periarteriolar [NO] significantly in a dose-dependent manner. ACh at 1 M increased [NO] from 438.1±43.4 nM at baseline to 647.9±66.3 nM, while 10 M of ACh increased [NO] from baseline to 1,035.0±59.2 nM (P<0.05). Neither 1 nor 10 M of ACh changed perivenular [NO] in the hamster cheek pouch. PAF, at 100 nM, increased perivenular [NO] from 326.6±50.8 to 622.8±41.5 nM. Importantly, 100 nM of PAF did not increase periarteriolar [NO]. PAF increased [NO] from 3.6±2.1 to 455.5±19.9 in CVEC, while ACh had no effect. Conclusions: We conclude that NO production can be stimulated in a differential manner in pre- and postcapillary segments in the hamster cheek pouch. ACh selectively stimulates the production of NO only in arterioles, while PAF stimulates the production of NO only in venules.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalMicrocirculation
Volume16
Issue number4
DOIs
StatePublished - 2009

Fingerprint

Cheek
Microvessels
Cricetinae
Nitric Oxide
Acetylcholine
Platelet Activating Factor
Venules
Arterioles
Microelectrodes
Microcirculation

Keywords

  • Acetylcholine
  • Arterioles
  • Microcirculation
  • Nitric oxide synthase
  • Platelet-activating factor
  • Regulation of microvascular function
  • Venules

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

Independent regulation of periarteriolar and perivenular nitric oxide mechanisms in the in vivo hamster cheek pouch microvasculature. / Kim, David D.; Kanetaka, Takehito; Durán, Ricardo G.; Sánchez, Fabiola A.; Bohlen, H.; Durán, Walter N.

In: Microcirculation, Vol. 16, No. 4, 2009, p. 323-330.

Research output: Contribution to journalArticle

Kim, David D. ; Kanetaka, Takehito ; Durán, Ricardo G. ; Sánchez, Fabiola A. ; Bohlen, H. ; Durán, Walter N. / Independent regulation of periarteriolar and perivenular nitric oxide mechanisms in the in vivo hamster cheek pouch microvasculature. In: Microcirculation. 2009 ; Vol. 16, No. 4. pp. 323-330.
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AB - Objective: We tested the hypothesis that differential stimulation of nitric oxide (NO) production can be induced in pre- and postcapillary segments of the microcirculation in the hamster cheek pouch. Materials and Methods: We applied acetylcholine (ACh) or platelet-activating factor (PAF) topically and measured perivascular NO concentration ([NO]) with NO-sensitive microelectrodes in arterioles and venules of the hamster cheek pouch. We also measured NO in cultured coronary endothelial cells (CVEC) after ACh or PAF. Results: ACh increased periarteriolar [NO] significantly in a dose-dependent manner. ACh at 1 M increased [NO] from 438.1±43.4 nM at baseline to 647.9±66.3 nM, while 10 M of ACh increased [NO] from baseline to 1,035.0±59.2 nM (P<0.05). Neither 1 nor 10 M of ACh changed perivenular [NO] in the hamster cheek pouch. PAF, at 100 nM, increased perivenular [NO] from 326.6±50.8 to 622.8±41.5 nM. Importantly, 100 nM of PAF did not increase periarteriolar [NO]. PAF increased [NO] from 3.6±2.1 to 455.5±19.9 in CVEC, while ACh had no effect. Conclusions: We conclude that NO production can be stimulated in a differential manner in pre- and postcapillary segments in the hamster cheek pouch. ACh selectively stimulates the production of NO only in arterioles, while PAF stimulates the production of NO only in venules.

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