Individual adult human neurons display aneuploidy: Detection by fluorescence in situ hybridization and single neuron PCR

Svetlana D. Pack, Robert J. Weil, Alexander O. Vortmeyer, Weifen Zeng, Jie Li, Hiroaki Okamoto, Makoto Furuta, Evgenia Pak, Irina A. Lubensky, Edward H. Oldfield, Zhengping Zhuang

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Neurons, once committed, exit the cell cycle and undergo maturation that promote specialized activity and are believed to operate upon a stable genome. We used fluorescence in situ hybridization, selective cell microdissection, and loss of heterozygosity analysis to assess degree of aneuploidy in patients with a neurodegenerative disease and in normal controls. We found that aneuploidy occurs in approximately 40% of mature, adult human neurons in health or disease and may be a physiological mechanism that maintains neuronal fate and function; it does not appear to be an unstable state. The fact that neuronal stem cells can be identified in adult humans and that somatic mosaicism may be found in neuronal precursor cells deserves further investigation before using adult neural stem cells to treat human disease.

Original languageEnglish (US)
Pages (from-to)1758-1760
Number of pages3
JournalCell Cycle
Volume4
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Aneuploidy
  • Fluorescence in situ hybridization
  • Neurons
  • Progenitor cells
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Pack, S. D., Weil, R. J., Vortmeyer, A. O., Zeng, W., Li, J., Okamoto, H., Furuta, M., Pak, E., Lubensky, I. A., Oldfield, E. H., & Zhuang, Z. (2005). Individual adult human neurons display aneuploidy: Detection by fluorescence in situ hybridization and single neuron PCR. Cell Cycle, 4(12), 1758-1760. https://doi.org/10.4161/cc.4.12.2153