Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells

Daniel Direnzo, David A. Hess, Barbara Damsz, Judy E. Hallett, Brett Marshall, Chirayu Goswami, Yunlong Liu, Tye Deering, Raymond J. MacDonald, Stephen F. Konieczny

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background & Aims: Early embryogenesis involves cell fate decisions that define the body axes and establish pools of progenitor cells. Development does not stop once lineages are specified; cells continue to undergo specific maturation events, and changes in gene expression patterns lead to their unique physiological functions. Secretory pancreatic acinar cells mature postnatally to synthesize large amounts of protein, polarize, and communicate with other cells. The transcription factor MIST1 is expressed by only secretory cells and regulates maturation events. MIST1-deficient acinar cells in mice do not establish apical-basal polarity, properly position zymogen granules, or communicate with adjacent cells, disrupting pancreatic function. We investigated whether MIST1 directly induces and maintains the mature phenotype of acinar cells. Methods: We analyzed the effects of Cre-mediated expression of Mist1 in adult Mist1-deficient (Mist1 KO) mice. Pancreatic tissues were collected and analyzed by light and electron microscopy, immunohistochemistry, real-time polymerase chain reaction analysis, and chromatin immunoprecipitation. Primary acini were isolated from mice and analyzed in amylase secretion assays. Results: Induced expression of Mist1 in adult Mist1 KO mice restored wild-type gene expression patterns in acinar cells. The acinar cells changed phenotypes, establishing apical-basal polarity, increasing the size of zymogen granules, reorganizing the cytoskeletal network, communicating intercellularly (by synthesizing gap junctions), and undergoing exocytosis. Conclusions: The exocrine pancreas of adult mice can be remodeled by re-expression of the transcription factor MIST1. MIST1 regulates acinar cell maturation and might be used to repair damaged pancreata in patients with pancreatic disorders.

Original languageEnglish
Pages (from-to)469-480
Number of pages12
JournalGastroenterology
Volume143
Issue number2
DOIs
StatePublished - Aug 2012

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Acinar Cells
Secretory Vesicles
Transcription Factors
Phenotype
Gene Expression
Exocrine Pancreas
Chromatin Immunoprecipitation
Gap Junctions
Exocytosis
Amylases
Embryonic Development
Real-Time Polymerase Chain Reaction
Pancreas
Electron Microscopy
Stem Cells
Immunohistochemistry
Light
Proteins

Keywords

  • DIMM
  • Exocrine Pancreas Disease
  • Secretion
  • Transcription

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Direnzo, D., Hess, D. A., Damsz, B., Hallett, J. E., Marshall, B., Goswami, C., ... Konieczny, S. F. (2012). Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells. Gastroenterology, 143(2), 469-480. https://doi.org/10.1053/j.gastro.2012.04.011

Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells. / Direnzo, Daniel; Hess, David A.; Damsz, Barbara; Hallett, Judy E.; Marshall, Brett; Goswami, Chirayu; Liu, Yunlong; Deering, Tye; MacDonald, Raymond J.; Konieczny, Stephen F.

In: Gastroenterology, Vol. 143, No. 2, 08.2012, p. 469-480.

Research output: Contribution to journalArticle

Direnzo, D, Hess, DA, Damsz, B, Hallett, JE, Marshall, B, Goswami, C, Liu, Y, Deering, T, MacDonald, RJ & Konieczny, SF 2012, 'Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells', Gastroenterology, vol. 143, no. 2, pp. 469-480. https://doi.org/10.1053/j.gastro.2012.04.011
Direnzo D, Hess DA, Damsz B, Hallett JE, Marshall B, Goswami C et al. Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells. Gastroenterology. 2012 Aug;143(2):469-480. https://doi.org/10.1053/j.gastro.2012.04.011
Direnzo, Daniel ; Hess, David A. ; Damsz, Barbara ; Hallett, Judy E. ; Marshall, Brett ; Goswami, Chirayu ; Liu, Yunlong ; Deering, Tye ; MacDonald, Raymond J. ; Konieczny, Stephen F. / Induced Mist1 expression promotes remodeling of mouse pancreatic acinar cells. In: Gastroenterology. 2012 ; Vol. 143, No. 2. pp. 469-480.
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