Inducible T Cell Antigen 4-1BB

Analysis of Expression and Function

Karen Pollok, Young June Kim, Zhen Zhou, José Hurtado, Kack K. Kim, Richard T. Pickard, Byoung S. Kwon

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

4-1BB is an inducible receptor-like protein expressed in both cytolytic and Th cells. Optimal induction of 4-1 BB mRNA in T cells required both PMA and ionomycin stimulation, indicating that protein kinase C activation and increases in intracellular Ca2+ were required for its expression. 4-1 BB was categorized as an early activation gene since the protein synthesis inhibitor, cycloheximide, blocked the induction of 4-1 BB mRNA. A rat mAb, 53A2, was generated against recombinant soluble 4-1 BB and was used to characterize this molecule. 4-1 BB is a 30-kDa glycoprotein and appears to exist as both a monomer and a 55-kDa dimer on the cell surface of a T cell clone. The 4-1 BB protein may be post-translationally modified since its predicted backbone is 25 kDa. FACS analysis indicated that 4-1 BB was inducible and expressed on the cell surface of activated splenic T cells and thymocytes. Cross-linking of 4-1BB on anti-CD3-stimulated T cells with 53A2 resulted in a dramatic enhancement of T cell proliferation. This suggests that 4-1BB may function as an accessory signaling molecule during T cell activation. Journal of Immunology, 1993, 150: 771.

Original languageEnglish
Pages (from-to)771-781
Number of pages11
JournalJournal of Immunology
Volume150
Issue number3
StatePublished - 1993

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Viral Tumor Antigens
T-Lymphocytes
Messenger RNA
Ionomycin
Proteins
Protein Synthesis Inhibitors
Thymocytes
Cycloheximide
Allergy and Immunology
Protein Kinase C
Glycoproteins
Clone Cells
Cell Proliferation

ASJC Scopus subject areas

  • Immunology

Cite this

Pollok, K., Kim, Y. J., Zhou, Z., Hurtado, J., Kim, K. K., Pickard, R. T., & Kwon, B. S. (1993). Inducible T Cell Antigen 4-1BB: Analysis of Expression and Function. Journal of Immunology, 150(3), 771-781.

Inducible T Cell Antigen 4-1BB : Analysis of Expression and Function. / Pollok, Karen; Kim, Young June; Zhou, Zhen; Hurtado, José; Kim, Kack K.; Pickard, Richard T.; Kwon, Byoung S.

In: Journal of Immunology, Vol. 150, No. 3, 1993, p. 771-781.

Research output: Contribution to journalArticle

Pollok, K, Kim, YJ, Zhou, Z, Hurtado, J, Kim, KK, Pickard, RT & Kwon, BS 1993, 'Inducible T Cell Antigen 4-1BB: Analysis of Expression and Function', Journal of Immunology, vol. 150, no. 3, pp. 771-781.
Pollok K, Kim YJ, Zhou Z, Hurtado J, Kim KK, Pickard RT et al. Inducible T Cell Antigen 4-1BB: Analysis of Expression and Function. Journal of Immunology. 1993;150(3):771-781.
Pollok, Karen ; Kim, Young June ; Zhou, Zhen ; Hurtado, José ; Kim, Kack K. ; Pickard, Richard T. ; Kwon, Byoung S. / Inducible T Cell Antigen 4-1BB : Analysis of Expression and Function. In: Journal of Immunology. 1993 ; Vol. 150, No. 3. pp. 771-781.
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