Inducible T cell antigen 4-1BB: Analysis of expression and function

K. E. Pollok, Y. J. Kim, Z. Zhou, J. Hurtado, K. K. Kim, R. T. Pickard, B. S. Kwon

Research output: Contribution to journalArticle

341 Scopus citations

Abstract

4-1BB is an inducible receptor-like protein expressed in both cytolytic and Th cells. Optimal induction of 4-1 BB mRNA in T cells required both PMA and ionomycin stimulation, indicating that protein kinase C activation and increases in intracellular Ca2+ were required for its expression. 4-1 BB was categorized as an early activation gene since the protein synthesis inhibitor, cycloheximide, blocked the induction of 4-1 BB mRNA. A rat mAb, 53A2, was generated against recombinant soluble 4-1 BB and was used to characterize this molecule. 4-1 BB is a 30-kDa glycoprotein and appears to exist as both a monomer and a 55-kDa dimer on the cell surface of a T cell clone. The 4-1 BB protein may be post-translationally modified since its predicted backbone is 25 kDa. FACS analysis indicated that 4-1 BB was inducible and expressed on the cell surface of activated splenic T cells and thymocytes. Cross-linking of 4-1BB on anti-CD3-stimulated T cells with 53A2 resulted in a dramatic enhancement of T cell proliferation. This suggests that 4-1BB may function as an accessory signaling molecule during T cell activation. Journal of Immunology, 1993, 150: 771.

Original languageEnglish (US)
Pages (from-to)771-781
Number of pages11
JournalJournal of Immunology
Volume150
Issue number3
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Pollok, K. E., Kim, Y. J., Zhou, Z., Hurtado, J., Kim, K. K., Pickard, R. T., & Kwon, B. S. (1993). Inducible T cell antigen 4-1BB: Analysis of expression and function. Journal of Immunology, 150(3), 771-781.