Induction and Expression of Amphiregulin in Human Pancreatic Cancer

Matthias Ebert, Munehiro Yokoyama, Michael S. Kobrin, Helmut Friess, Martha E. Lopez, Markus W. Büchler, Gibbes R. Johnson, Murray Korc

Research output: Contribution to journalArticle

142 Scopus citations

Abstract

The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregulin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor α. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.

Original languageEnglish (US)
Pages (from-to)3959-3962
Number of pages4
JournalCancer Research
Volume54
Issue number15
StatePublished - Aug 1994

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ebert, M., Yokoyama, M., Kobrin, M. S., Friess, H., Lopez, M. E., Büchler, M. W., Johnson, G. R., & Korc, M. (1994). Induction and Expression of Amphiregulin in Human Pancreatic Cancer. Cancer Research, 54(15), 3959-3962.