Induction and expression of amphiregulin in human pancreatic cancer

Matthias Ebert, Munehiro Yokoyama, Michael S. Kobrin, Helmut Friess, Martha E. Lopez, Markus W. Büchler, Gibbes R. Johnson, Murray Korc

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregulin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor α. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.

Original languageEnglish (US)
Pages (from-to)3959-3962
Number of pages4
JournalCancer Research
Volume54
Issue number15
StatePublished - Aug 1 1994
Externally publishedYes

Fingerprint

Pancreatic Neoplasms
Epidermal Growth Factor Receptor
Cell Line
Transforming Growth Factors
Amphiregulin
Cell Nucleus
Northern Blotting
Disease Progression
Pancreas
Intercellular Signaling Peptides and Proteins
Cytoplasm
Acetates
Carcinoma
Polymerase Chain Reaction
Messenger RNA
Peptides
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ebert, M., Yokoyama, M., Kobrin, M. S., Friess, H., Lopez, M. E., Büchler, M. W., ... Korc, M. (1994). Induction and expression of amphiregulin in human pancreatic cancer. Cancer Research, 54(15), 3959-3962.

Induction and expression of amphiregulin in human pancreatic cancer. / Ebert, Matthias; Yokoyama, Munehiro; Kobrin, Michael S.; Friess, Helmut; Lopez, Martha E.; Büchler, Markus W.; Johnson, Gibbes R.; Korc, Murray.

In: Cancer Research, Vol. 54, No. 15, 01.08.1994, p. 3959-3962.

Research output: Contribution to journalArticle

Ebert, M, Yokoyama, M, Kobrin, MS, Friess, H, Lopez, ME, Büchler, MW, Johnson, GR & Korc, M 1994, 'Induction and expression of amphiregulin in human pancreatic cancer', Cancer Research, vol. 54, no. 15, pp. 3959-3962.
Ebert M, Yokoyama M, Kobrin MS, Friess H, Lopez ME, Büchler MW et al. Induction and expression of amphiregulin in human pancreatic cancer. Cancer Research. 1994 Aug 1;54(15):3959-3962.
Ebert, Matthias ; Yokoyama, Munehiro ; Kobrin, Michael S. ; Friess, Helmut ; Lopez, Martha E. ; Büchler, Markus W. ; Johnson, Gibbes R. ; Korc, Murray. / Induction and expression of amphiregulin in human pancreatic cancer. In: Cancer Research. 1994 ; Vol. 54, No. 15. pp. 3959-3962.
@article{06272622b131428694b60350206f7da2,
title = "Induction and expression of amphiregulin in human pancreatic cancer",
abstract = "The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregulin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor α. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.",
author = "Matthias Ebert and Munehiro Yokoyama and Kobrin, {Michael S.} and Helmut Friess and Lopez, {Martha E.} and B{\"u}chler, {Markus W.} and Johnson, {Gibbes R.} and Murray Korc",
year = "1994",
month = "8",
day = "1",
language = "English (US)",
volume = "54",
pages = "3959--3962",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "15",

}

TY - JOUR

T1 - Induction and expression of amphiregulin in human pancreatic cancer

AU - Ebert, Matthias

AU - Yokoyama, Munehiro

AU - Kobrin, Michael S.

AU - Friess, Helmut

AU - Lopez, Martha E.

AU - Büchler, Markus W.

AU - Johnson, Gibbes R.

AU - Korc, Murray

PY - 1994/8/1

Y1 - 1994/8/1

N2 - The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregulin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor α. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.

AB - The epidermal growth factor receptor is activated by a family of polypeptides that includes the growth factor amphiregulin (AR). Using Northern blot analysis and the polymerase chain reaction, we now report that AR mRNA is expressed in human pancreatic cancer cell lines, and that this expression is enhanced in several of these cell lines by tetradecanoyl phorbol acetate and transforming growth factor α. AR was also expressed in normal and malignant pancreatic tissues. However, in the normal pancreas, AR immunostaining was most evident in the nuclei of ductal cells. In contrast, in many carcinomas, AR was also present in the cytoplasm of the ductal-like cancer cells. Cytoplasmic localization of AR was associated with a more advanced clinical stage. These findings suggest that AR may contribute to aberrant activation of the epidermal growth factor receptor in human pancreatic cancer, and may enhance disease progression.

UR - http://www.scopus.com/inward/record.url?scp=0028101982&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028101982&partnerID=8YFLogxK

M3 - Article

C2 - 8033121

AN - SCOPUS:0028101982

VL - 54

SP - 3959

EP - 3962

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 15

ER -