Induction and expression of heparin-binding EGF-like growth factor in human pancreatic cancer

Michael S. Kobrin, Hitoshi Funatomi, Helmut Friess, Markus W. Buchler, Peter Stathis, Murray Korc

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor. We examined HB-EGF biological action and expression in human pancreatic cancer cell lines, and compared HB-EGF expression in normal and cancerous pancreatic tissues. HB-EGF enhanced the growth of human pancreatic cancer cells in a dose-dependent manner. Several cell lines expressed HB-EGF mRNA transcripts, and the transcript level was enhanced by HB-EGF, as well as by 12-O-tetradecanoylphorbol-13-acetate and transforming growth factor-alpha (TGF-α). By comparison with the normal pancreas, HB-EGF mRNA levels were increased in human pancreatic cancer tissues. These findings suggest that HB-EGF may participate in aberrant autocrine and paracrine activation of the EGF receptor, thereby contributing to pancreatic cancer cell growth.

Original languageEnglish (US)
Pages (from-to)1705-1709
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number3
DOIs
StatePublished - Aug 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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