Induction and Expression of Heparin-Binding EGF-Like Growth Factor in Human Pancreatic Cancer

M. S. Kobrin, H. Funatomi, H. Friess, M. W. Buchler, P. Stathis, Murray Korc

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor. We examined HB-EGF biological action and expression in human pancreatic cancer cell lines, and compared HB-EGF expression in normal and cancerous pancreatic tissues. HB-EGF enhanced the growth of human pancreatic cancer cells in a dose-dependent manner. Several cell lines expressed HB-EGF mRNA transcripts, and the transcript level was enhanced by HB-EGF, as well as by 12-O-tetradecanoylphorbol-13-acetate and transforming growth factor-alpha (TGF-α). By comparison with the normal pancreas, HB-EGF mRNA levels were increased in human pancreatic cancer tissues. These findings suggest that HB-EGF may participate in aberrant autocrine and paracrine activation of the EGF receptor, thereby contributing to pancreatic cancer cell growth.

Original languageEnglish (US)
Pages (from-to)1705-1709
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number3
DOIs
StatePublished - Aug 15 1994
Externally publishedYes

Fingerprint

Pancreatic Neoplasms
Epidermal Growth Factor
Heparin
Intercellular Signaling Peptides and Proteins
Cells
Epidermal Growth Factor Receptor
Tissue
Cell Line
Messenger RNA
Transforming Growth Factor alpha
Cell growth
Tetradecanoylphorbol Acetate
Growth
Heparin-binding EGF-like Growth Factor
Pancreas
Acetates
Molecular Weight
Chemical activation
Molecular weight
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Induction and Expression of Heparin-Binding EGF-Like Growth Factor in Human Pancreatic Cancer. / Kobrin, M. S.; Funatomi, H.; Friess, H.; Buchler, M. W.; Stathis, P.; Korc, Murray.

In: Biochemical and Biophysical Research Communications, Vol. 202, No. 3, 15.08.1994, p. 1705-1709.

Research output: Contribution to journalArticle

Kobrin, M. S. ; Funatomi, H. ; Friess, H. ; Buchler, M. W. ; Stathis, P. ; Korc, Murray. / Induction and Expression of Heparin-Binding EGF-Like Growth Factor in Human Pancreatic Cancer. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 202, No. 3. pp. 1705-1709.
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