Induction of β-lactamase activity and decreased β-lactam susceptibility by CO2 in clinical bacterial isolates

Nathan Mullen, Hugo Raposo, Polyxeni Gudis, Linsey Barker, Romney M. Humphries, Bryan Schmitt, Ryan Relich, Meghan May

Research output: Contribution to journalArticle

Abstract

Antimicrobial susceptibility testing of clinical isolates is a crucial step toward appropriate treatment of infectious diseases. The clinical isolate Francisella philomiragia 14IUHPL001, recently isolated from a 63-year-old woman with atypical pneumonia, featured decreased susceptibility to β-lactam antibiotics when cultivated in 5% CO2. Quantitative β-lactamase assays demonstrated a significant (P < 0.0001) increase in enzymatic activity between bacteria cultivated in 5% CO2 over those incubated in ambient air. The presence of β-lactamase genes blaTEM and blaSHV was detected in the clinical isolate F. philomiragia 14IUHPL001 by PCR, and the genes were positively identified by nucleotide sequencing. Expression of blaTEM and blaSHV was detected by reverse transcription-PCR during growth at 5% CO2 but not during growth in ambient air. A statistically significant alkaline shift was observed following cultivation of F. philomiragia 14IUHPL001 in both ambient air and 5% CO2, allowing desegregation of the previously reported effects of acidic pH from the currently reported effect of 5% CO2 on blaTEM and blaSHV β-lactamases. To ensure that the observed phenomenon was not unique to F. philomiragia, we evaluated a clinical isolate of blaTEM-carrying Haemophilus influenzae and found parallel induction of blaTEM gene expression and β-lactamase activity at 5% CO2 relative to ambient air.

Original languageEnglish (US)
Article numbere00266-17
JournalmSphere
Volume2
Issue number4
DOIs
StatePublished - Jul 1 2017

Fingerprint

Lactams
Air
Francisella
Polymerase Chain Reaction
Haemophilus influenzae
Growth
Genes
Reverse Transcription
Communicable Diseases
Pneumonia
Nucleotides
Anti-Bacterial Agents
Bacteria
Gene Expression
Therapeutics

Keywords

  • Antimicrobial resistance
  • BLA
  • Carbon dioxide
  • Francisella
  • Haemophilus
  • SHV
  • TEM
  • β-lactamases

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Induction of β-lactamase activity and decreased β-lactam susceptibility by CO2 in clinical bacterial isolates. / Mullen, Nathan; Raposo, Hugo; Gudis, Polyxeni; Barker, Linsey; Humphries, Romney M.; Schmitt, Bryan; Relich, Ryan; May, Meghan.

In: mSphere, Vol. 2, No. 4, e00266-17, 01.07.2017.

Research output: Contribution to journalArticle

Mullen, Nathan ; Raposo, Hugo ; Gudis, Polyxeni ; Barker, Linsey ; Humphries, Romney M. ; Schmitt, Bryan ; Relich, Ryan ; May, Meghan. / Induction of β-lactamase activity and decreased β-lactam susceptibility by CO2 in clinical bacterial isolates. In: mSphere. 2017 ; Vol. 2, No. 4.
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