Induction of cellular growth by the axon guidance regulators netrin A and semaphorin-1a

Ellen Flannery, Adrienne VanZomeren-Dohm, Paul Beach, Wendy Simanton Holland, Molly Scheel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although neurite outgrowth has been linked to axon guidance regulators, the effects of guidance molecules on cellular growth are not well understood. Use of the Drosophila wing imaginal disc, an epithelial tissue and a well-characterized system for analysis of cellular growth regulation, permits analysis of the impacts of guidance molecules on cellular growth in a setting in which axon guidance is not a confounding factor. In this investigation, the impacts of Netrin A (NetA) and Semaphorin-1a (Sema1a) signaling on cellular growth are examined during wing development. Levels of these genes were modulated in somatic clones in the developing wing disc, and clone areas, as well as individual sizes of clonal cells were assessed. NetA and Sema1a signaling were found to induce cellular growth in these assays. Furthermore, immunohistochemical analyses indicated that NetA and Sema1a signaling induce expression of several growth regulators, including myc, cycD, cdk4, PCNA, and MapK in the wing disc. These data illustrate that NetA and Sema1a can specifically promote growth through induction of key cellular growth regulators. The abilities of NetA and Sema1a to regulate cellular growth are likely critical to their functions in both nervous system development and oncogenesis.

Original languageEnglish
Pages (from-to)473-484
Number of pages12
JournalDevelopmental Neurobiology
Volume70
Issue number7
DOIs
StatePublished - Jun 2010

Fingerprint

Semaphorins
Growth
Clone Cells
Imaginal Discs
Axon Guidance
Proliferating Cell Nuclear Antigen
Systems Analysis
Cell Size
Nervous System
Drosophila
Carcinogenesis
Epithelium

Keywords

  • Development
  • Drosophila
  • Growth
  • Netrin
  • Semaphorin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Induction of cellular growth by the axon guidance regulators netrin A and semaphorin-1a. / Flannery, Ellen; VanZomeren-Dohm, Adrienne; Beach, Paul; Holland, Wendy Simanton; Scheel, Molly.

In: Developmental Neurobiology, Vol. 70, No. 7, 06.2010, p. 473-484.

Research output: Contribution to journalArticle

Flannery, Ellen ; VanZomeren-Dohm, Adrienne ; Beach, Paul ; Holland, Wendy Simanton ; Scheel, Molly. / Induction of cellular growth by the axon guidance regulators netrin A and semaphorin-1a. In: Developmental Neurobiology. 2010 ; Vol. 70, No. 7. pp. 473-484.
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