Induction of enoyl-CoA hydratase by LD50 exposure to perfluorocarboxylic acids detected by two-dimensional electrophoresis

Frank Witzmann, D. N. Parker, B. M. Jarnot

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of in vivo exposure to perfluoro-n-octanoic and perfluoro-n-decanoic acids was examined in the rat liver by two-dimensional electrophoresis (2DE). Using nonequilibrium pH-gradient electrophoresis in the first dimension separation, proteins associated with the mitochondrial/peroxisomal cell fraction were observed and immunologically identified. Conspicuous inductions in peroxisomal enoyl-CoA hydratase and other proteins of the peroxisomal β-oxidative pathway were observed following single-dose exposure to each compound. The abundance of the tentatively-identified mitochondrial equivalent, crotonase, was not altered by these intoxications. These results confirm previous observations of perfluorocarboxylic acid toxicity and support the use of 2D protein-pattern alterations in biomarker research. The ability to identify this type of alteration via 2DE, in association with specific toxic effects by chemically related compounds, may provide new and additional markers for chemical-induced tissue damage.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalToxicology Letters
Volume71
Issue number3
DOIs
StatePublished - 1994

Fingerprint

Enoyl-CoA Hydratase
Lethal Dose 50
Electrophoresis
Acids
Proteins
Proton-Motive Force
Poisons
Biomarkers
Liver
Toxicity
Rats
Association reactions
Tissue
Research

Keywords

  • Crotonase
  • Liver
  • Nonequilibrium pH-gradient electrophoresis
  • Peroxisomes
  • Two-dimensional electrophoresis
  • β-Oxidation

ASJC Scopus subject areas

  • Toxicology

Cite this

Induction of enoyl-CoA hydratase by LD50 exposure to perfluorocarboxylic acids detected by two-dimensional electrophoresis. / Witzmann, Frank; Parker, D. N.; Jarnot, B. M.

In: Toxicology Letters, Vol. 71, No. 3, 1994, p. 271-277.

Research output: Contribution to journalArticle

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N2 - The effect of in vivo exposure to perfluoro-n-octanoic and perfluoro-n-decanoic acids was examined in the rat liver by two-dimensional electrophoresis (2DE). Using nonequilibrium pH-gradient electrophoresis in the first dimension separation, proteins associated with the mitochondrial/peroxisomal cell fraction were observed and immunologically identified. Conspicuous inductions in peroxisomal enoyl-CoA hydratase and other proteins of the peroxisomal β-oxidative pathway were observed following single-dose exposure to each compound. The abundance of the tentatively-identified mitochondrial equivalent, crotonase, was not altered by these intoxications. These results confirm previous observations of perfluorocarboxylic acid toxicity and support the use of 2D protein-pattern alterations in biomarker research. The ability to identify this type of alteration via 2DE, in association with specific toxic effects by chemically related compounds, may provide new and additional markers for chemical-induced tissue damage.

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