Induction of heat shock protein 70 mRNA in adult hamster facial nuclear groups following axotomy of the facial nerve

Geraldine A. New, Brian R. Hendrickson, Kathryn Jones

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In this study, the effects of axotomy on heat shock protein 70 (hsp70) mRNA levels were analyzed by northern blot hybridization with a mouse-inducible hsp70 cDNA probe. The right facial nerve of adult golden hamsters was axotomized external to the skull, with the left nerve serving as sham-operated and internal control. Postoperative survival times were 0.5, 2, 6, 12, and 24 hr, with three to six animals per time point. Total RNA in the axotomized and control facial nuclear groups, dissected from the brain stem, was isolated by guanidinium-thiocyanate extraction and analyzed by northern hybridization and scanning densitometry. For normalization, the blots were rehybridized with a genomic cDNA probe complementary to the 28 S rRNA. At 0.5 hr postoperative, there was a 68% induction of hsp70 mRNA levels as compared to control. This increase peaked at 2 hr postoperative with a 280% induction and declined subsequently to 160% at 6 hr, 33% at 12 hr, and - 56% at 24 hr, relative to control levels. As a positive control, hyperthermia-induced hsp70 expression was established in hamster whole brain using northern blot hybridization and the mouse-inducible hsp70 cDNA probe. There was a 126% increase in hsp70 mRNA levels relative to control at 1.5 hr following hyperthermia. By 6.5 hr after the 1.5-hr hyperthermic period, there was a 40% decrease in hsp70 mRNA levels below control. The results provide the first evidence that peripheral axotomy acts as a cell stressor to induce the expression of hsp70 mRNA. Within our current understanding of the nerve cell body response to injury, the data represent the earliest changes in gene expression following axotomy reported thus far.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalMetabolic Brain Disease
Volume4
Issue number4
DOIs
StatePublished - Dec 1989
Externally publishedYes

Fingerprint

Axotomy
HSP70 Heat-Shock Proteins
Facial Nerve
Cricetinae
Messenger RNA
Complementary DNA
Level control
Northern Blotting
Brain
Induced Hyperthermia
Densitometry
Mesocricetus
Skull
Gene expression
Brain Stem
Neurons
Animals
Fever
RNA
Scanning

Keywords

  • axotomy
  • heat shock
  • neuronal injury
  • regeneration

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Biochemistry

Cite this

Induction of heat shock protein 70 mRNA in adult hamster facial nuclear groups following axotomy of the facial nerve. / New, Geraldine A.; Hendrickson, Brian R.; Jones, Kathryn.

In: Metabolic Brain Disease, Vol. 4, No. 4, 12.1989, p. 273-279.

Research output: Contribution to journalArticle

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abstract = "In this study, the effects of axotomy on heat shock protein 70 (hsp70) mRNA levels were analyzed by northern blot hybridization with a mouse-inducible hsp70 cDNA probe. The right facial nerve of adult golden hamsters was axotomized external to the skull, with the left nerve serving as sham-operated and internal control. Postoperative survival times were 0.5, 2, 6, 12, and 24 hr, with three to six animals per time point. Total RNA in the axotomized and control facial nuclear groups, dissected from the brain stem, was isolated by guanidinium-thiocyanate extraction and analyzed by northern hybridization and scanning densitometry. For normalization, the blots were rehybridized with a genomic cDNA probe complementary to the 28 S rRNA. At 0.5 hr postoperative, there was a 68{\%} induction of hsp70 mRNA levels as compared to control. This increase peaked at 2 hr postoperative with a 280{\%} induction and declined subsequently to 160{\%} at 6 hr, 33{\%} at 12 hr, and - 56{\%} at 24 hr, relative to control levels. As a positive control, hyperthermia-induced hsp70 expression was established in hamster whole brain using northern blot hybridization and the mouse-inducible hsp70 cDNA probe. There was a 126{\%} increase in hsp70 mRNA levels relative to control at 1.5 hr following hyperthermia. By 6.5 hr after the 1.5-hr hyperthermic period, there was a 40{\%} decrease in hsp70 mRNA levels below control. The results provide the first evidence that peripheral axotomy acts as a cell stressor to induce the expression of hsp70 mRNA. Within our current understanding of the nerve cell body response to injury, the data represent the earliest changes in gene expression following axotomy reported thus far.",
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