Induction of human choriogonadotropin and follitropin in HeLa cell cultures by hyperosmolality

Robert Fallon, R. P. Cox, N. K. Ghosh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The growth of cervical carcinoma cell (HeLa) cultures in a hyperosmolar environment stimulates increased production of the onco-developmental peptides human choriogonadotropin (hCG) and follitropin (FSH). This effect was observed in two sublines examined in this study, HeLa65 and HeLa71. hCG and FSH were measured by radioimmunoassay using antiserum against the β-subunit of the hormone dimer, the insuring immunochemical specificity. The amounts of hCG and FSH produced by HeLa65 an HeLa71 cells cultured in hyperosmolar medium were 2- to 50-fold higher than corresponding hormone levels in basal cultures. Synthesis of gonadotropins dependened on concentration and duration of exposure the hyperosmolar medium. Levels of culture medium osmolality effective in inducing hormone production also inhibit the incorporation of 14C-thymidine into acid-insoluble macromolecules. Hyperosmolality thus stimulates the ectopic production of gonadotropic hormones while retarding cellular growth and nucleic acid synthesis.

Original languageEnglish (US)
Pages (from-to)613-618
Number of pages6
JournalJournal of Cellular Physiology
Volume98
Issue number3
StatePublished - 1979
Externally publishedYes

Fingerprint

Follicle Stimulating Hormone
Chorionic Gonadotropin
HeLa Cells
Cell culture
Cell Culture Techniques
Hormones
Human Follicle Stimulating Hormone
Growth
Gonadotropins
Macromolecules
Dimers
Osmolar Concentration
Thymidine
Nucleic Acids
Radioimmunoassay
Culture Media
Immune Sera
Cultured Cells
Carcinoma
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Induction of human choriogonadotropin and follitropin in HeLa cell cultures by hyperosmolality. / Fallon, Robert; Cox, R. P.; Ghosh, N. K.

In: Journal of Cellular Physiology, Vol. 98, No. 3, 1979, p. 613-618.

Research output: Contribution to journalArticle

Fallon, Robert ; Cox, R. P. ; Ghosh, N. K. / Induction of human choriogonadotropin and follitropin in HeLa cell cultures by hyperosmolality. In: Journal of Cellular Physiology. 1979 ; Vol. 98, No. 3. pp. 613-618.
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