Induction of MCP-1 expression in airway epithelial cells: Role of CCR2 receptor in airway epithelial injury

Matthew C. Lundien, Kamal A. Mohammed, Najmunnisa Nasreen, R. S. Tepper, Joyce A. Hardwick, Kerry L. Sanders, Robert D. Van Horn, Veena B. Antony

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The repair of an injured bronchial epithelial cell (BEC) monolayer requires proliferation and migration of BECs into the injured area. We hypothesized that BEC monolayer injury results in monocyte chemoattractant protein-1 (MCP-1) production, which initiates the repair process. BECs (BEAS-2B from ATCC) were utilized in this study. MCP-1 interacts with CCR2B receptor (CCR2B), resulting in cell proliferation, haptotaxis, and healing of the monolayer. Reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to verify the presence of CCR2B. CCR2B was not merely present but also inducible by interleukin-2 (IL-2) and lipopolysaccharide (LPS). We demonstrated by immunohistochemistry that BECs express MCP-1 after injury and that receptor expression can be regulated by exposure to IL-2 and LPS. Haptotactic migration of cells was enhanced in the presence of MCP-1 and reduced in the presence of CCR2B antibody. This enhanced or depressed ability of the BECs to perform haptotactic migration was shown to be statistically significant (P < 0.05) when compared to controls. Finally, BECs proliferate in response to MCP-1 as proven by electric cell-substrate impedance sensing (ECIS) technology. MCP-1-specific antibodies were shown to neutralize the MCP-1-mediated BEC proliferation. This cascade of events following injury to the bronchial epithelium may provide insight into the mechanism of the repair process.

Original languageEnglish (US)
Pages (from-to)144-152
Number of pages9
JournalJournal of Clinical Immunology
Volume22
Issue number3
DOIs
StatePublished - Jun 17 2002

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CCR2 Receptors
Chemokine CCL2
Epithelial Cells
Wounds and Injuries
Interleukin-2
Lipopolysaccharides
Cell Proliferation
Aptitude
Antibodies
Chemotaxis
Reverse Transcriptase Polymerase Chain Reaction
Electric Impedance
Cell Movement
Epithelium
Immunohistochemistry
Technology

Keywords

  • Bronchial epithelial cells
  • CCR2B
  • Chemokines
  • Haptotaxis
  • IL-2
  • Lipopolysaccharide
  • MCP-1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Induction of MCP-1 expression in airway epithelial cells : Role of CCR2 receptor in airway epithelial injury. / Lundien, Matthew C.; Mohammed, Kamal A.; Nasreen, Najmunnisa; Tepper, R. S.; Hardwick, Joyce A.; Sanders, Kerry L.; Van Horn, Robert D.; Antony, Veena B.

In: Journal of Clinical Immunology, Vol. 22, No. 3, 17.06.2002, p. 144-152.

Research output: Contribution to journalArticle

Lundien, MC, Mohammed, KA, Nasreen, N, Tepper, RS, Hardwick, JA, Sanders, KL, Van Horn, RD & Antony, VB 2002, 'Induction of MCP-1 expression in airway epithelial cells: Role of CCR2 receptor in airway epithelial injury', Journal of Clinical Immunology, vol. 22, no. 3, pp. 144-152. https://doi.org/10.1023/A:1015420029430
Lundien, Matthew C. ; Mohammed, Kamal A. ; Nasreen, Najmunnisa ; Tepper, R. S. ; Hardwick, Joyce A. ; Sanders, Kerry L. ; Van Horn, Robert D. ; Antony, Veena B. / Induction of MCP-1 expression in airway epithelial cells : Role of CCR2 receptor in airway epithelial injury. In: Journal of Clinical Immunology. 2002 ; Vol. 22, No. 3. pp. 144-152.
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