Induction of MIC-1/growth differentiation factor-15 following bile duct injury

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Macrophage inflammatory peptide-1 (MIC-1)/growth/differentiation factor-15 (GDF-15) is a divergent member of the transforming growth factor-β superfamily cloned by others and us. MIC-1/GDF-15 is expressed in the liver, breast, and colon. Studies have demonstrated a growth-inhibiting effect of MIC-1/GDF-15 on colon and breast cancer cell lines in vitro and on tumor growth in vivo. We previously reported that MIC-1 expression is rapidly induced after a wide variety of murine acute and chronic liver injuries including aniline dye administration. I hypothesized, therefore, that MIC-1/GDF-15 may be a mediator of biliary tract injury and could play a role in regulation of bile duct proliferation. C57BL/6 mice underwent surgical ligation of the common bile duct. Northern blot analysis revealed a time-dependent induction of MIC-1/GDF-15 mRNA in the liver. In situ hybridization of liver sections for MIC-1/GDF-15 expression after bile duct ligation demonstrated a zone 1 or periportal expression pattern, consistent with expression of MIC-1 in periductular hepatocytes. Northern blot analysis of liver mRNA from patients with sclerosing cholangitis or cirrhosis also demonstrated enhanced expression of MIC-1/GDF-15. MIC-1/GDF-15 is expressed after bile duct injury in mice and humans. Taken together with the previously demonstrated growth inhibitory effects of MIC-1/GDF-15 on normal and transformed cells, MIC-1/GDF-15 may play a role in regulation of bile duct proliferation and biliary tumor formation.

Original languageEnglish (US)
Pages (from-to)901-905
Number of pages5
JournalJournal of Gastrointestinal Surgery
Volume7
Issue number7
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Growth Differentiation Factor 15
Bile Ducts
Wounds and Injuries
Liver
Northern Blotting
Ligation
Growth
Sclerosing Cholangitis
Messenger RNA
Common Bile Duct
Biliary Tract
Transforming Growth Factors
Inbred C57BL Mouse
Colonic Neoplasms
In Situ Hybridization
Hepatocytes
Neoplasms
Colon
Breast

Keywords

  • Cholangiocarcinoma
  • Cytokines
  • Growth substances
  • Inflammation

ASJC Scopus subject areas

  • Surgery

Cite this

Induction of MIC-1/growth differentiation factor-15 following bile duct injury. / Koniaris, Leonidas.

In: Journal of Gastrointestinal Surgery, Vol. 7, No. 7, 11.2003, p. 901-905.

Research output: Contribution to journalArticle

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