Induction of mitotic S-phase of host and neighboring cells by Toxoplasma gondii enhances parasite invasion

Mark D. Lavine, Gustavo Arrizabalaga

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The intracellular parasite Toxoplasma gondii extensively modifies its host cell so as to efficiently grow and divide. Among these cellular changes, T. gondii alters the cell cycle of host cells it has invaded. We found that T. gondii affects the cell cycle of not only the cells it directly invades, but neighboring cells as well. Both direct invasion by T. gondii and exposure to filtered medium from cultures of T. gondii-infected cells (conditioned medium) caused normally quiescent fibroblasts to enter S-phase. T. gondii has been shown to attach to and invade S-phase host cells more readily, and we found that conditioned medium increased the rate of invasion of T. gondii into new host cells. Thus it appears that T. gondii directly releases, or induces parasitized host cells to release, a factor that induces neighboring cells to enter S-phase, allowing more rapid invasion by extracellular T. gondii and providing a possible selective advantage for the parasite.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalMolecular and Biochemical Parasitology
Volume164
Issue number1
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Toxoplasma
S Phase
Parasites
Conditioned Culture Medium
Cell Cycle
Culture Media
Fibroblasts

Keywords

  • BrdU
  • Cell cycle
  • Invasion
  • S-phase
  • Toxoplasma

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Induction of mitotic S-phase of host and neighboring cells by Toxoplasma gondii enhances parasite invasion. / Lavine, Mark D.; Arrizabalaga, Gustavo.

In: Molecular and Biochemical Parasitology, Vol. 164, No. 1, 03.2009, p. 95-99.

Research output: Contribution to journalArticle

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