Induction of neurofibrillary tangles in cultured mouse neurons by maytanprine

Yuji Sato, Seung U. Kim, Bernardino Ghetti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neurofibrillary (neurofilament) tangles were induced in cultured neurons of newborn mouse dorsal root ganglia by exposure to maytanprine, an anti-microtubule drug. By 12-24 h of treatment, small short neurofilament bundles were found in the neuronal perikarya and proximal portions of the neurites. By 48-72 h of treatment, small neurofilament bundles were assembled into larger masses, and after 72 h and onward several neurofilament bundles coalesced into single large distinctive perinuclear rings in the neuronal perikarya. A histometric analysis of maytanprine treated axons demonstrated that the density of microtubules decreased rapidly, while that of neurofilaments decreased more slowly. As a result of microtubule depolymerization by maytanprine treatment, neurofilaments change their distribution pattern in neuronal perikarya and subsequently form tangles.

Original languageEnglish
Pages (from-to)191-203
Number of pages13
JournalJournal of the Neurological Sciences
Volume68
Issue number2-3
DOIs
StatePublished - 1985

Fingerprint

Neurofibrillary Tangles
Intermediate Filaments
Neurons
Microtubules
Spinal Ganglia
Neurites
Axons
maytanprine
Pharmaceutical Preparations

Keywords

  • Antimicrotubule drug
  • Culture
  • Maytanprine
  • Microtubules
  • Mouse
  • Neurofibrillary tangles
  • Neurofilaments
  • Neurons

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Cite this

Induction of neurofibrillary tangles in cultured mouse neurons by maytanprine. / Sato, Yuji; Kim, Seung U.; Ghetti, Bernardino.

In: Journal of the Neurological Sciences, Vol. 68, No. 2-3, 1985, p. 191-203.

Research output: Contribution to journalArticle

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