Induction of neurofibrillary tangles in cultured mouse neurons by maytanprine

Yuji Sato, Seung U. Kim, Bernardino Ghetti

Research output: Contribution to journalArticle

10 Scopus citations


Neurofibrillary (neurofilament) tangles were induced in cultured neurons of newborn mouse dorsal root ganglia by exposure to maytanprine, an anti-microtubule drug. By 12-24 h of treatment, small short neurofilament bundles were found in the neuronal perikarya and proximal portions of the neurites. By 48-72 h of treatment, small neurofilament bundles were assembled into larger masses, and after 72 h and onward several neurofilament bundles coalesced into single large distinctive perinuclear rings in the neuronal perikarya. A histometric analysis of maytanprine treated axons demonstrated that the density of microtubules decreased rapidly, while that of neurofilaments decreased more slowly. As a result of microtubule depolymerization by maytanprine treatment, neurofilaments change their distribution pattern in neuronal perikarya and subsequently form tangles.

Original languageEnglish (US)
Pages (from-to)191-203
Number of pages13
JournalJournal of the Neurological Sciences
Issue number2-3
StatePublished - May 1985


  • Antimicrotubule drug
  • Culture
  • Maytanprine
  • Microtubules
  • Mouse
  • Neurofibrillary tangles
  • Neurofilaments
  • Neurons

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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