Induction of osteoblastic cell differentiation by forskolin. Stimulation of cyclic AMP production and alkaline phosphatase activity

Yoshiyuki Hakeda, Eiko Ikeda, Noriyoshi Kurihara, Yoshinori Nakatani, Norihiko Maeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The effects of forskolin on differentiation of osteoblastic cells (clone MC3T3-E1) cultured in α-minimum essential medium containing 0.1% bovine serum albumin were investigated by assays of intracellular cyclic AMP level and alkaline phosphatase activity in the cells. Forskolin increased cyclic AMP production in the cells in a dose-related manner, the maximum increase being 250-fold above that of the controls. Alkaline phosphatase activity in the cells was also elevated as early as 24 h and rose to nearly its maximum at 48 h. The elevation was dose-dependent, with a maximum increase at 5 · 10-6 M forskolin. Forskolin and prostaglandin E2 showed a supraadditive effect on cyclic AMP production in the cells and had an additive effect on alkaline phosphatase activity, whereas forskolin and dibutyryl cyclic AMP had little additive effect on either on cyclic AMP production or enzyme activity. These results suggest that cyclic AMP is closely linked to the differentiation of osteoblastic cells in vivo.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalBBA - General Subjects
Volume838
Issue number1
DOIs
StatePublished - Jan 28 1985
Externally publishedYes

Keywords

  • (Osteoblast)
  • Alkaline phosphatase
  • Differentiation
  • Forskolin
  • cyclic AMP synthesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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