Induction of pulmonary immunity in cattle by oral administration of ovalbumin in alginate microspheres

Terry L. Bowersock, Harm HogenEsch, Sandra Torregrosa, Della Borie, Ben Wang, Haesun Park, Kinam Park

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Respiratory infectious diseases are an important cause of economic losses to the cattle industry. There is a need for an effective, easy to administer vaccine to the critical bacterial pathogens that cause pneumonia in cattle. An orally administered vaccine could be given to a large number of animals without significant stress to the animals and with minimal labor. The purpose of this study was to determine whether the oral administration of a model antigen (ovalbumin) in alginate microspheres could induce pulmonary immunity in cattle. Calves were vaccinated orally with ovalbumin (OVA) following either a subcutaneous (SC) or oral priming dose of OVA. Calves primed and boostered by oral administration (oral/oral) of OVA encapsulated in alginate microparticles had increased numbers of antigen-specific IgA ASCs (ASCs) in bronchoalveolar lavage (BAL) fluids. Calves that received a SC priming followed by an oral booster inoculation (SC/oral) of OVA in alginate microspheres had a greater number of anti-OVA IgA, IgG1 and IgG2 ASCs in BALF. SC/oral calves also had increased numbers of anti-OVA IgG1 ASCs in peripheral blood whereas oral/oral calves had none. SC/oral calves had increased anti-OVA IgG1, IgG2, and IgA titers in BALF, and IgG1 and IgG2 in serum compared to both oral/oral and sham vaccinated calves. These results indicate that oral administration of antigen encapsulated in alginate microspheres results in a mucosal immune response in the respiratory tract of cattle. Furthermore, SC priming both enhanced the IgA response and stimulated an IgG1 and IgG2 response not seen in oral/oral calves. The difference in antibody isotype results suggest that design of the vaccination protocol can direct antibody responses as needed for a specific immunization program.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalImmunology Letters
Volume60
Issue number1
DOIs
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Ovalbumin
Microspheres
Oral Administration
Immunity
Immunoglobulin G
Lung
Immunoglobulin A
Antigens
Vaccines
alginic acid
Immunization Programs
Mucosal Immunity
Bronchoalveolar Lavage Fluid
Respiratory System
Antibody Formation
Communicable Diseases
Pneumonia
Industry
Vaccination
Economics

Keywords

  • Alginate
  • IgA
  • Microspheres
  • Mucosal immunity
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Induction of pulmonary immunity in cattle by oral administration of ovalbumin in alginate microspheres. / Bowersock, Terry L.; HogenEsch, Harm; Torregrosa, Sandra; Borie, Della; Wang, Ben; Park, Haesun; Park, Kinam.

In: Immunology Letters, Vol. 60, No. 1, 01.1998, p. 37-43.

Research output: Contribution to journalArticle

Bowersock, Terry L. ; HogenEsch, Harm ; Torregrosa, Sandra ; Borie, Della ; Wang, Ben ; Park, Haesun ; Park, Kinam. / Induction of pulmonary immunity in cattle by oral administration of ovalbumin in alginate microspheres. In: Immunology Letters. 1998 ; Vol. 60, No. 1. pp. 37-43.
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