Induction of steady-state blood alcohol levels: Application to the study of within-session alcohol tolerance in rats

Janice Froehlich, R. B. Stewart, T. K. Li, A. K. Mosemiller, D. E. McCullough, M. C. Ho, J. M. Kisner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The study of within-session alcohol tolerance in the rat has been hampered by methodological difficulties related to the measurement of dependent variables at predictable blood alcohol concentrations (BAC) during a single session of alcohol exposure. This study characterizes a method for maintaining steady-state blood alcohol levels over several hours in the rat, referred to as the "alcohol clamp." Methods: Wistar rats were implanted with an indwelling catheter in the carotid artery for blood sampling and another in the external jugular vein for alcohol infusion. To clamp BAC at a predetermined level, rats first were infused with a priming dose of alcohol to establish the desired or "target" BAC, followed by a continuous infusion of alcohol at a rate equal to that of alcohol metabolism in the rat. This maintained BAC at a constant level over time. BACs of 100, 200, or 300 mg% were maintained over several hours in separate groups of rats. The alcohol clamp was applied to the study of acute (within-session) alcohol tolerance in rats selectively bred for high and low alcohol drinking. Alcohol-induced hypothermia was used to index tolerance, and within-session alcohol tolerance was defined as a return of body temperature toward baseline during the course of the alcohol infusion while BAC was maintained at a constant level. Results: The continuous alcohol infusion procedure maintained BAC in a steady state throughout the 3 hr alcohol infusion session at each of the three target BAC levels. Alcohol infusion induced a drop in body temperature, followed by a return of temperature toward baseline during the course of infusion, which indicated the development of within-session alcohol tolerance. Conclusions: The continuous alcohol infusion procedure (alcohol clamp) maintained BAC in a steady state, both within and between subjects, across a wide range of blood alcohol levels. The alcohol clamp appears to be a useful tool for subsequent studies of within-session alcohol tolerance in the rat.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume25
Issue number3
StatePublished - 2001

Fingerprint

Rats
Blood
Alcohols
Clamping devices
Blood Alcohol Content
Body Temperature
Induced Hypothermia
Indwelling Catheters
Jugular Veins
Carotid Arteries
Alcohol Drinking
Hypothermia
Wistar Rats

Keywords

  • Blood Alcohol Concentration
  • Steady State

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Froehlich, J., Stewart, R. B., Li, T. K., Mosemiller, A. K., McCullough, D. E., Ho, M. C., & Kisner, J. M. (2001). Induction of steady-state blood alcohol levels: Application to the study of within-session alcohol tolerance in rats. Alcoholism: Clinical and Experimental Research, 25(3), 370-376.

Induction of steady-state blood alcohol levels : Application to the study of within-session alcohol tolerance in rats. / Froehlich, Janice; Stewart, R. B.; Li, T. K.; Mosemiller, A. K.; McCullough, D. E.; Ho, M. C.; Kisner, J. M.

In: Alcoholism: Clinical and Experimental Research, Vol. 25, No. 3, 2001, p. 370-376.

Research output: Contribution to journalArticle

Froehlich, Janice ; Stewart, R. B. ; Li, T. K. ; Mosemiller, A. K. ; McCullough, D. E. ; Ho, M. C. ; Kisner, J. M. / Induction of steady-state blood alcohol levels : Application to the study of within-session alcohol tolerance in rats. In: Alcoholism: Clinical and Experimental Research. 2001 ; Vol. 25, No. 3. pp. 370-376.
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