Induction of systemic and mucosal immune response in cattle by intranasal administration of pig serum albumin in alginate microparticles

M. C. Rebelatto, P. Guimond, T. L. Bowersock, H. HogenEsch

Research output: Contribution to journalArticle

52 Scopus citations


Biodegradable microparticles are an efficient mucosal delivery system that protect antigens from the harsh mucosal environment and facilitate their uptake by M cells at the epithelium of mucosal-associated lymphoid tissue. In this study, we determined the systemic and mucosal immune response in calves following intranasal and oral immunization with pig serum albumin (PSA) encapsulated in alginate microparticles. The size of the particles ranged from 1 to 50μm in diameter, with 95% of the particles being smaller than 5μm. High levels of anti-PSA IgG1 antibodies were found in the serum, nasal secretions, and to a less extent in saliva of calves vaccinated intranasally, but not orally, with PSA-microparticles. There was no significant increase of PSA-specific IgA. A weak lymphocyte proliferative immune response was observed in peripheral blood mononuclear cells (PBMCs), and few anti-PSA antibody-secreting cells (ASC) were detected in the blood of calves immunized intranasally. The combined systemic and mucosal response observed in intranasally immunized animals may be attributed to the wide variation in the size of the alginate microparticles, with smaller particles translocating to regional lymph nodes and inducing a systemic immune response, and larger particles being retained in the NALT and inducing a mucosal immune response. The procedure presented here may be useful as an intranasal vaccine against respiratory diseases in cattle.

Original languageEnglish (US)
Pages (from-to)93-105
Number of pages13
JournalVeterinary Immunology and Immunopathology
Issue number1-2
StatePublished - Oct 16 2001
Externally publishedYes


  • Alginate
  • Bovine
  • Intranasal vaccine
  • Microspheres
  • Mucosal immunity

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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