Inductive effects of prostaglandins on alkaline phosphatase in osteoblastic cells, clone MC3T3-E1

Yoshiyuki Hakeda, Yoshinori Nakatani, Masahiko Hiramatsu, Noriyoshi Kurihara, Mayuri Tsunol, Eiko Ikeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

The effects of prostaglandins (PGs) on the induction of alkaline phosphatase (ALP) were investigated in osteoblastic clone MC3T3-E1 cells cultured in serum-free medium. Prostaglandin E2 (PGE2) stimulated ALP activity in the cells in a dose-dependent fashion with a maximal effect which was about twice that in the control cells at concentrations of 100-500 ng/ml. Actinomycin D and cycloheximide inhibited the stimulative effect of PGE2, on ALP activity in the cells. PGE2,-induced and native ALPs in the cells were of the same type as that in adult mouse calvaria, being heat-labile, L-homoarginine- and levamisole-sensitive, and L-phenylalanine-insensitive. Isobutyl methylxanthine (IBMX), a cAMP phosphodiesterase inhibitor, stimulated the inductive effect of PGE2 on ALP activity at 0.1 mM, at which concentration IBMX alone had little effect on the activity. PGE2 also increased the intracellular cAMP content in a dose-dependent fashion with a maximal effect at 100 ng/ml. PGE1, PGF1a, and PGF2a, (primary PGs like PGE2) increased the activity. Our present results suggest that PGs stimulate the differentiation of osteoblasts and are involved in bone formation in vivo, as well as in bone resorption.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalJournal of Biochemistry
Volume97
Issue number1
DOIs
StatePublished - Jan 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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