Infection of human cells with murine amphotropic replication-competent retroviruses

Kenneth Cornetta, Nga Nguyen, Richard A. Morgan, Daryl D. Muenchau, Janet W. Hartley, R. Michael Blaese, W. French Anderson

Research output: Contribution to journalArticle

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Abstract

Replication of the murine wild-type 4070A amphotropic retrovirus and a recombinant amphotropic replication-competent retrovirus arising from the PA12 packaging cell line varied considerably among the primate cell types tested. Medium from infected primate fibroblasts and endothelial cells contained the highest viral titers [104-105 focus-forming units (ffu)/ml], while most hematopoietic cell lines, such as K562 and MOLT4, were associated with viral titers in the range of 103-104 ffu/ml. Interestingly, HTLV-1-transformed T cell lines (TJF-2 and HM) and primary tumor infiltrating lymphocytes (TIL) had very low viral titer (0-101 ffu/ml). The low production of virus was not due to low infectivity and, in contrast to the virus, retroviral vectors were expressed without difficulty. Because screening for replication-competent retrovirus (RCR) is an important component of human retroviral-mediated gene therapy clinical protocols, a variety of assays were tested for their ability to detect RCR in virus-exposed cell lines. A biologic assay (3T3 amplification) and polymerase chain reaction (PCR) for the 4070A viral envelope are effective screening methods for RCR, even in cell lines associated with low virus production.

Original languageEnglish
Pages (from-to)579-588
Number of pages10
JournalHuman Gene Therapy
Volume4
Issue number5
StatePublished - Oct 1993

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Retroviridae
Viruses
Cell Line
Infection
Primates
Tumor-Infiltrating Lymphocytes
Transformed Cell Line
Human T-lymphotropic virus 1
Product Packaging
Clinical Protocols
Biological Assay
Genetic Therapy
Endothelial Cells
Fibroblasts
T-Lymphocytes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Cornetta, K., Nguyen, N., Morgan, R. A., Muenchau, D. D., Hartley, J. W., Blaese, R. M., & Anderson, W. F. (1993). Infection of human cells with murine amphotropic replication-competent retroviruses. Human Gene Therapy, 4(5), 579-588.

Infection of human cells with murine amphotropic replication-competent retroviruses. / Cornetta, Kenneth; Nguyen, Nga; Morgan, Richard A.; Muenchau, Daryl D.; Hartley, Janet W.; Blaese, R. Michael; Anderson, W. French.

In: Human Gene Therapy, Vol. 4, No. 5, 10.1993, p. 579-588.

Research output: Contribution to journalArticle

Cornetta, K, Nguyen, N, Morgan, RA, Muenchau, DD, Hartley, JW, Blaese, RM & Anderson, WF 1993, 'Infection of human cells with murine amphotropic replication-competent retroviruses', Human Gene Therapy, vol. 4, no. 5, pp. 579-588.
Cornetta K, Nguyen N, Morgan RA, Muenchau DD, Hartley JW, Blaese RM et al. Infection of human cells with murine amphotropic replication-competent retroviruses. Human Gene Therapy. 1993 Oct;4(5):579-588.
Cornetta, Kenneth ; Nguyen, Nga ; Morgan, Richard A. ; Muenchau, Daryl D. ; Hartley, Janet W. ; Blaese, R. Michael ; Anderson, W. French. / Infection of human cells with murine amphotropic replication-competent retroviruses. In: Human Gene Therapy. 1993 ; Vol. 4, No. 5. pp. 579-588.
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