Influence of age and fetal hypophysectomy on porcine preadipocytes: Insulin-like growth factor-I (IGF-I) response, receptor binding and IGF binding proteins secretion

Xuening (Neal) Chen, G. J. Hausman, J. T. Wright

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effects of fetal hypophysectomy (hypox) and age on insulin-like growth factor-I (IGF-I) response, receptor binding and IGF-I and IGF binding protein secretion by porcine preadipocytes was examined. Primary cultures of stromal vascular (S-V) cells derived from fetal and postnatal adipose tissue were established and exposed to test media (different concentrations of pig serum with or without IGF-I) for morphological study or to serum-free media for measuring IGF-I receptor binding and IGF-I and IGFBPs secretion. Conditioned media were collected and subjected to IGF-I RIA and ligand blot and immunoblot analysis. IGF-I significantly stimulated total cell growth (determined by DNA levels) and increased fat cell cluster number and lipid deposition in porcine S-V cultures (determined by image analysis). Although the pattern of response to IGF-I was similar in fetal and postnatal cultures, the response was greater in fetal cultures. The mitogenic and adipogenic response of fetal preadipocytes to IGF-I was impaired by fetal hypox. Specific IGF-I binding sites on fetal preadipocytes had a higher binding affinity for IGF-I (K(d) = 2.82 ± 0.28 nM) than did postnatal preadipocytes (K(d) = 7.28 ± 1.52 nM, P <0.05). Preadipocytes secreted IGF-I and four IGFBPs (BP-1, -2, -3, and -4) in a developmentally regulated manner. For example, IGFBP-2, and -3 were predominant binding proteins secreted by fetal preadipocytes which represented 47% and 30% of the total, respectively. IGFBP-1 was the major binding protein secreted by postnatal preadipocytes. Furthermore, fetal cells secreted greater amounts of IGF-I in cultures than did postnatal cells (2428 ± 205 pg/ml vs. 870 ± 31 pg/ml, P <0.05). Fetal hypox caused a decrease from 47% to 68% of control values in all four IGFBPs secreted by preadipocytes. The changes in IGF-I receptor binding and IGF-I and IGFBP secretion by preadipocytes may account for altered response of preadipocytes to IGF-I. Thus, secretion of IGF-I and IGFBPs, IGF-I binding and response of porcine preadipocytes were affected by age and fetal hypox. The results indicated that locally produced IGF-I and IGFBPs may play a critical paracrine/autocrine role during fetal adipose development.

Original languageEnglish (US)
Pages (from-to)193-206
Number of pages14
JournalGrowth, Development and Aging
Volume59
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Insulin, Regular, Pork
hypophysectomy
Insulin-Like Growth Factor Binding Proteins
Hypophysectomy
IGF Type 1 Receptor
insulin-like growth factor binding proteins
protein secretion
insulin-like growth factor I
Insulin-Like Growth Factor I
Gestational Age
receptors
swine
Swine
Insulin-Like Growth Factor Binding Protein 1
secretion
blood vessels
Blood Vessels
Carrier Proteins
binding proteins
Insulin-Like Growth Factor Binding Protein 2

Keywords

  • Development
  • Fetal hypophysectomy
  • Fetal pigs
  • IGF-I
  • IGFBPs
  • Preadipocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

@article{d04ca38673914347a5e8c2aaf2bfdd99,
title = "Influence of age and fetal hypophysectomy on porcine preadipocytes: Insulin-like growth factor-I (IGF-I) response, receptor binding and IGF binding proteins secretion",
abstract = "The effects of fetal hypophysectomy (hypox) and age on insulin-like growth factor-I (IGF-I) response, receptor binding and IGF-I and IGF binding protein secretion by porcine preadipocytes was examined. Primary cultures of stromal vascular (S-V) cells derived from fetal and postnatal adipose tissue were established and exposed to test media (different concentrations of pig serum with or without IGF-I) for morphological study or to serum-free media for measuring IGF-I receptor binding and IGF-I and IGFBPs secretion. Conditioned media were collected and subjected to IGF-I RIA and ligand blot and immunoblot analysis. IGF-I significantly stimulated total cell growth (determined by DNA levels) and increased fat cell cluster number and lipid deposition in porcine S-V cultures (determined by image analysis). Although the pattern of response to IGF-I was similar in fetal and postnatal cultures, the response was greater in fetal cultures. The mitogenic and adipogenic response of fetal preadipocytes to IGF-I was impaired by fetal hypox. Specific IGF-I binding sites on fetal preadipocytes had a higher binding affinity for IGF-I (K(d) = 2.82 ± 0.28 nM) than did postnatal preadipocytes (K(d) = 7.28 ± 1.52 nM, P <0.05). Preadipocytes secreted IGF-I and four IGFBPs (BP-1, -2, -3, and -4) in a developmentally regulated manner. For example, IGFBP-2, and -3 were predominant binding proteins secreted by fetal preadipocytes which represented 47{\%} and 30{\%} of the total, respectively. IGFBP-1 was the major binding protein secreted by postnatal preadipocytes. Furthermore, fetal cells secreted greater amounts of IGF-I in cultures than did postnatal cells (2428 ± 205 pg/ml vs. 870 ± 31 pg/ml, P <0.05). Fetal hypox caused a decrease from 47{\%} to 68{\%} of control values in all four IGFBPs secreted by preadipocytes. The changes in IGF-I receptor binding and IGF-I and IGFBP secretion by preadipocytes may account for altered response of preadipocytes to IGF-I. Thus, secretion of IGF-I and IGFBPs, IGF-I binding and response of porcine preadipocytes were affected by age and fetal hypox. The results indicated that locally produced IGF-I and IGFBPs may play a critical paracrine/autocrine role during fetal adipose development.",
keywords = "Development, Fetal hypophysectomy, Fetal pigs, IGF-I, IGFBPs, Preadipocytes",
author = "Chen, {Xuening (Neal)} and Hausman, {G. J.} and Wright, {J. T.}",
year = "1995",
language = "English (US)",
volume = "59",
pages = "193--206",
journal = "Growth, Development and Aging",
issn = "1041-1232",
publisher = "Growth Publishing Co. Inc.",
number = "4",

}

TY - JOUR

T1 - Influence of age and fetal hypophysectomy on porcine preadipocytes

T2 - Insulin-like growth factor-I (IGF-I) response, receptor binding and IGF binding proteins secretion

AU - Chen, Xuening (Neal)

AU - Hausman, G. J.

AU - Wright, J. T.

PY - 1995

Y1 - 1995

N2 - The effects of fetal hypophysectomy (hypox) and age on insulin-like growth factor-I (IGF-I) response, receptor binding and IGF-I and IGF binding protein secretion by porcine preadipocytes was examined. Primary cultures of stromal vascular (S-V) cells derived from fetal and postnatal adipose tissue were established and exposed to test media (different concentrations of pig serum with or without IGF-I) for morphological study or to serum-free media for measuring IGF-I receptor binding and IGF-I and IGFBPs secretion. Conditioned media were collected and subjected to IGF-I RIA and ligand blot and immunoblot analysis. IGF-I significantly stimulated total cell growth (determined by DNA levels) and increased fat cell cluster number and lipid deposition in porcine S-V cultures (determined by image analysis). Although the pattern of response to IGF-I was similar in fetal and postnatal cultures, the response was greater in fetal cultures. The mitogenic and adipogenic response of fetal preadipocytes to IGF-I was impaired by fetal hypox. Specific IGF-I binding sites on fetal preadipocytes had a higher binding affinity for IGF-I (K(d) = 2.82 ± 0.28 nM) than did postnatal preadipocytes (K(d) = 7.28 ± 1.52 nM, P <0.05). Preadipocytes secreted IGF-I and four IGFBPs (BP-1, -2, -3, and -4) in a developmentally regulated manner. For example, IGFBP-2, and -3 were predominant binding proteins secreted by fetal preadipocytes which represented 47% and 30% of the total, respectively. IGFBP-1 was the major binding protein secreted by postnatal preadipocytes. Furthermore, fetal cells secreted greater amounts of IGF-I in cultures than did postnatal cells (2428 ± 205 pg/ml vs. 870 ± 31 pg/ml, P <0.05). Fetal hypox caused a decrease from 47% to 68% of control values in all four IGFBPs secreted by preadipocytes. The changes in IGF-I receptor binding and IGF-I and IGFBP secretion by preadipocytes may account for altered response of preadipocytes to IGF-I. Thus, secretion of IGF-I and IGFBPs, IGF-I binding and response of porcine preadipocytes were affected by age and fetal hypox. The results indicated that locally produced IGF-I and IGFBPs may play a critical paracrine/autocrine role during fetal adipose development.

AB - The effects of fetal hypophysectomy (hypox) and age on insulin-like growth factor-I (IGF-I) response, receptor binding and IGF-I and IGF binding protein secretion by porcine preadipocytes was examined. Primary cultures of stromal vascular (S-V) cells derived from fetal and postnatal adipose tissue were established and exposed to test media (different concentrations of pig serum with or without IGF-I) for morphological study or to serum-free media for measuring IGF-I receptor binding and IGF-I and IGFBPs secretion. Conditioned media were collected and subjected to IGF-I RIA and ligand blot and immunoblot analysis. IGF-I significantly stimulated total cell growth (determined by DNA levels) and increased fat cell cluster number and lipid deposition in porcine S-V cultures (determined by image analysis). Although the pattern of response to IGF-I was similar in fetal and postnatal cultures, the response was greater in fetal cultures. The mitogenic and adipogenic response of fetal preadipocytes to IGF-I was impaired by fetal hypox. Specific IGF-I binding sites on fetal preadipocytes had a higher binding affinity for IGF-I (K(d) = 2.82 ± 0.28 nM) than did postnatal preadipocytes (K(d) = 7.28 ± 1.52 nM, P <0.05). Preadipocytes secreted IGF-I and four IGFBPs (BP-1, -2, -3, and -4) in a developmentally regulated manner. For example, IGFBP-2, and -3 were predominant binding proteins secreted by fetal preadipocytes which represented 47% and 30% of the total, respectively. IGFBP-1 was the major binding protein secreted by postnatal preadipocytes. Furthermore, fetal cells secreted greater amounts of IGF-I in cultures than did postnatal cells (2428 ± 205 pg/ml vs. 870 ± 31 pg/ml, P <0.05). Fetal hypox caused a decrease from 47% to 68% of control values in all four IGFBPs secreted by preadipocytes. The changes in IGF-I receptor binding and IGF-I and IGFBP secretion by preadipocytes may account for altered response of preadipocytes to IGF-I. Thus, secretion of IGF-I and IGFBPs, IGF-I binding and response of porcine preadipocytes were affected by age and fetal hypox. The results indicated that locally produced IGF-I and IGFBPs may play a critical paracrine/autocrine role during fetal adipose development.

KW - Development

KW - Fetal hypophysectomy

KW - Fetal pigs

KW - IGF-I

KW - IGFBPs

KW - Preadipocytes

UR - http://www.scopus.com/inward/record.url?scp=0029616027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029616027&partnerID=8YFLogxK

M3 - Article

C2 - 8770611

AN - SCOPUS:0029616027

VL - 59

SP - 193

EP - 206

JO - Growth, Development and Aging

JF - Growth, Development and Aging

SN - 1041-1232

IS - 4

ER -