Influence of enamel thickness on quantification of mineral loss in enamel using laser-induced fluorescence

Masatoshi Ando, B. R. Schemehorn, G. J. Eckert, D. T. Zero, G. K. Stookey

Research output: Contribution to journalArticle

11 Scopus citations


The objective of this study was to evaluate the effect of enamel thickness on the quantification of white-spot lesions with laser fluorescence. One hundred and twenty 3-mm-diameter human ground and polished enamel specimens were used. Specimens were illuminated by a 488-nm argon laser, images were acquired through a 540-nm high-pass filter and stored on the Quantitative Light-Induced Fluorescence (QLF) program. Specimens were divided into two groups and demineralized for 48 or 96 h and QLF images were again acquired. Fluorescence radiance (FR) of both sound (FRs) and demineralized (FRd) enamel was determined using the QLF program. Change in fluorescence radiance (ΔFR, %) was determined as follows: ΔFR = FRd/FRs × 100. One thin section was obtained for transverse microradiography analysis. Enamel and dentin thickness of the thin sections were measured. There was a good correlation between QLF parameters and enamel thickness. It can be concluded that the FR observed for similar lesions depends on the actual enamel thickness.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalCaries research
Issue number1
StatePublished - Jan 1 2003


  • Dentin
  • Enamel
  • Fluorescence
  • Laser
  • Microradiography
  • White spots

ASJC Scopus subject areas

  • Dentistry(all)

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