Influence of IL-1α and -1β on the survival of human bone marrow cells responding to hematopoietic colony-stimulating factors

G. Hangoc, D. E. Williams, J. H F Falkenburg, Hal Broxmeyer

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Abstract

Purified recombinant human (rhu) IL-1α and IL-1β were evaluated for their effects on the proliferation and survival of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells from normal human bone marrow (BM). Using nonadherent low density T lymphocyte depleted (NALT-) BM cells cultured in the presence or absence of IL-1, CSF-deprivation studies demonstrated that IL-1α or IL-1β by itself did not enhance the proliferation of CFU-GM or BFU-E. They did, however, promote the survival of progenitors responding to the delayed addition of media conditioned by the 5637 cell line (5637 conditioned medium), rhu GM-CSF and erythropoietin. The survival promoting effects of IL-1α on CFU-GM and BFU-E were neutralized by anti-IL-1α mAb added to the cultures. The survival promoting effect of IL-1α did not appear to be mediated by CSF, because neither CSF nor erythroid burst promoting activity were detectable in cultures in which NALT- cells were incubated with rhuIL-1α. In addition, suboptimal concentrations of rhu macrophage CSF (CSF-1), G-CSF, GM-CSF, and IL-3, which were just below the levels that would stimulate colony formation, did not enhance progenitor cell survival. Survival of CFU-GM and BFU-E in low density (LD) bone marrow cells did not decrease as drastically as that in NALT- BM cells, and exogenously added IL-1 did not enhance progenitor cell survival of CFU-GM and BFU-E in LD BM cells. However, addition of anti-IL-1β decreased survival of CFU-GM and BFU-E in LD BM cells. These results implicate IL-1 in the prolonged survival of human CFU-GM and BFU-E.

Original languageEnglish
Pages (from-to)4329-4334
Number of pages6
JournalJournal of Immunology
Volume142
Issue number12
StatePublished - 1989

Fingerprint

Colony-Stimulating Factors
Interleukin-1
Bone Marrow Cells
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Stem Cells
Conditioned Culture Medium
Cell Survival
Macrophages
Macrophage Colony-Stimulating Factor
Survival
Interleukin-3
Granulocyte Colony-Stimulating Factor
Erythropoietin
Granulocytes
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Influence of IL-1α and -1β on the survival of human bone marrow cells responding to hematopoietic colony-stimulating factors. / Hangoc, G.; Williams, D. E.; Falkenburg, J. H F; Broxmeyer, Hal.

In: Journal of Immunology, Vol. 142, No. 12, 1989, p. 4329-4334.

Research output: Contribution to journalArticle

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