Influence of interleukins 3, 5, and 6 on the growth of eosinophil progenitors in highly enriched human bone marrow in the absence of serum

L. Lu, Z. H. Lin, R. N. Shen, D. J. Warren, T. Leemhuis, Hal Broxmeyer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purified preparations of natural murine interleukin (IL)-5 and recombinant human IL-1α, IL-3, and IL-6 were evaluated alone, and in combination, for effects in vitro on colony formation by eosinophil progenitors (eosinophil colony-forming units, CFU-Eos) in either nonadherent low-density T-lymphocyte depleted (NALT-) or fluorescence-activated cells sorted NALT- HLA-DR+ CD34 (My10)+++ cells from normal bone marrow, in the absence and presence of serum. Interleukin were assessed on CFU-Eos plated directly in agar, or on CFY-Eos placed in suspension culture for 7 days prior to plating in agar in the presence of IL-5. Eosinophil colonies were identified in situ by staining with Luxol fast blue. IL-3 and IL-5 acted in agar culture as direct stimulators of CFU-Eos, using highly purified cells in the HLA-DR+ My10+++ fraction of cells, the specificity for CFU-Eos being greatest for IL-5 and most demonstrable in the absence of serum. The IL-3 and IL-5 direct stimulating effects on CFU-Eos were additive. IL-1α and IL-6 had little or no effect, alone or in combination with IL-3 and IL-5; however, IL-3, IL-5, and IL-6 each enhanced the number of IL-5-responsive CFU-Eos found after suspension culture compared to control medium, with the individual effects being additive when the molecules were combined. These results demonstrate that both IL-3 and IL-5, alone and in combination, have direct-acting effects on CFU-Eos, with the greatest specificity for stimulation of pure Eos colonies and clusters residing in IL-5, and that IL-3, IL-5, and IL-6, alone and in combination may play a role in the survival of CFU-Eos.

Original languageEnglish
Pages (from-to)1180-1186
Number of pages7
JournalExperimental Hematology
Volume18
Issue number11
StatePublished - 1990

Fingerprint

Interleukin-3
Interleukin-5
Eosinophils
Interleukin-6
Bone Marrow
Growth
Serum
Agar
HLA-DR Antigens
Interleukin-1
Suspensions
T-Lymphocytes
Interleukins
Bone Marrow Cells
Stem Cells
Fluorescence
Staining and Labeling

Keywords

  • Eosinophil progenitors
  • Interleukin 5

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Influence of interleukins 3, 5, and 6 on the growth of eosinophil progenitors in highly enriched human bone marrow in the absence of serum. / Lu, L.; Lin, Z. H.; Shen, R. N.; Warren, D. J.; Leemhuis, T.; Broxmeyer, Hal.

In: Experimental Hematology, Vol. 18, No. 11, 1990, p. 1180-1186.

Research output: Contribution to journalArticle

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