Influence of mitoxantrone on nucleolar function in MDA-MB-231 human breast tumor cell line

Nasser Chegini, Ahmad R. Safa

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effect of mitoxantrone (DHAQ) on [3H]thymidine and [3H]uridine incorporation by exponentially growing MDA-MB-231, a human breast tumor cell line has been studied. The results have indicated that DHAQ was more effective in inhibiting [3H]thymidine than [3H]uridine incorporation in a concentration dependent manner. Following drug treatment at 20 ng/ml concentration, 50% inhibition of growth and [3H]thymidine incorporation were noted, whereas [3H]uridine incorporation was only inhibited by about 12%. At 2000 ng/ml of DHAQ the inhibition of cell growth, [3H]thymidine and [3H]uridine incorporations were 78%, 95% and 62%, respectively. Nuclear-associated radioactivity detected at light and electron microscope autoradiographic levels after [3H]thymidine and [3H]uridine incorporations, into DHAQ treated cells indicated that DHAQ prevented the accumulation of radioactivity into the nuclei in a concentration dependent manner. These results gave further indication that mitoxantrone induced a definitive alteration of nuclear template activities, correlated with nuclear functional-structural relation and suggested that the nucleoli were the primary site of DHAQ action.

Original languageEnglish (US)
Pages (from-to)327-336
Number of pages10
JournalCancer Letters
Volume37
Issue number3
DOIs
StatePublished - Nov 1987
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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