Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells

H. E. Broxmeyer, G. Hangoc, S. Cooper, D. Anderson, D. Cosman, S. D. Lyman, D. E. Williams

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Purified natural and recombinant murine mast cell growth factor (MGF, a c-kit ligand) were evaluated alone and in combination with other cytokines for effects in vitro on colony formation by multipotential (GFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells from BDF1 mouse bone marrow. Both preparations stimulated Epo-dependent CFU-GEMM and enhanced Epo-dependent BFU-E colony numbers and size. MGF had some stimulating activity for CFU-GM. When used in combination with plateau concentrations of pokeweed mitogen mouse spleen cell conditioned medium or granulocyte-macrophage colony stimulating factor (CSF), MGF enhanced in greater than additive fashion colony formation by CFU-GM. MGF also enhanced the size of colonies formed, an enhancement greatest for colonies containing granulocytes and macrophages. MGF did not enhance macrophage-CSF stimulated colony numbers or size. MGF seems to be an early acting cytokine with preferential effects on the growth of more immature hematopoietic progenitor cells.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalExperimental Hematology
Volume19
Issue number2
StatePublished - Mar 20 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Broxmeyer, H. E., Hangoc, G., Cooper, S., Anderson, D., Cosman, D., Lyman, S. D., & Williams, D. E. (1991). Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells. Experimental Hematology, 19(2), 143-146.