Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells

Hal Broxmeyer, G. Hangoc, S. Cooper, D. Anderson, D. Cosman, S. D. Lyman, D. E. Williams

Research output: Contribution to journalArticle

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Abstract

Purified natural and recombinant murine mast cell growth factor (MGF, a c-kit ligand) were evaluated alone and in combination with other cytokines for effects in vitro on colony formation by multipotential (GFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells from BDF1 mouse bone marrow. Both preparations stimulated Epo-dependent CFU-GEMM and enhanced Epo-dependent BFU-E colony numbers and size. MGF had some stimulating activity for CFU-GM. When used in combination with plateau concentrations of pokeweed mitogen mouse spleen cell conditioned medium or granulocyte-macrophage colony stimulating factor (CSF), MGF enhanced in greater than additive fashion colony formation by CFU-GM. MGF also enhanced the size of colonies formed, an enhancement greatest for colonies containing granulocytes and macrophages. MGF did not enhance macrophage-CSF stimulated colony numbers or size. MGF seems to be an early acting cytokine with preferential effects on the growth of more immature hematopoietic progenitor cells.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalExperimental Hematology
Volume19
Issue number2
StatePublished - 1991

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Granulocyte-Macrophage Progenitor Cells
Stem Cell Factor
Hematopoietic Stem Cells
Erythroid Precursor Cells
Bone Marrow
Granulocytes
Macrophages
Cytokines
Myeloid Progenitor Cells
Pokeweed Mitogens
Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Conditioned Culture Medium
Stem Cells
Spleen
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Broxmeyer, H., Hangoc, G., Cooper, S., Anderson, D., Cosman, D., Lyman, S. D., & Williams, D. E. (1991). Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells. Experimental Hematology, 19(2), 143-146.

Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells. / Broxmeyer, Hal; Hangoc, G.; Cooper, S.; Anderson, D.; Cosman, D.; Lyman, S. D.; Williams, D. E.

In: Experimental Hematology, Vol. 19, No. 2, 1991, p. 143-146.

Research output: Contribution to journalArticle

Broxmeyer, H, Hangoc, G, Cooper, S, Anderson, D, Cosman, D, Lyman, SD & Williams, DE 1991, 'Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells', Experimental Hematology, vol. 19, no. 2, pp. 143-146.
Broxmeyer, Hal ; Hangoc, G. ; Cooper, S. ; Anderson, D. ; Cosman, D. ; Lyman, S. D. ; Williams, D. E. / Influence of murine mast cell growth factor (c-kit ligand) on colony formation by mouse marrow hematopoietic progenitor cells. In: Experimental Hematology. 1991 ; Vol. 19, No. 2. pp. 143-146.
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