Influence of periostin-positive cell-specific Klf5 deletion on aortic thickening in DOCA-salt hypertensive mice

Hirofumi Zempo, Jun Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Katsuhito Fujiu, Ichiro Manabe, Simon J. Conway, Yoshiaki Taniyama, Ryuichi Morishita, Yasunobu Hirata, Mitsuaki Isobe, Ryozo Nagai

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Chronic hypertension causes vascular remodeling that is associated with an increase in periostin- (postn) positive cells, including fibroblasts and smooth muscle cells. Krüppel-like factor (KLF) 5, a transcription factor, is also observed in vascular remodeling; however, it is unknown what role KLF5 plays in postn-positive cells during vascular remodeling induced by deoxycorticosterone-acetate (DOCA) salt. We used postn-positive cell-specific Klf5-deficient mice (Klf5 Postn KO: Klf5 flox/flox; Postn Cre/-) and wild-type mice (WT: Klf5 flox/flox; Postn -/-). We implanted a DOCA pellet and provided drinking water containing 0.9% NaCl for 8 weeks. The DOCA-salt treatment induced hypertension in both genotypes, as observed by increases in systolic blood pressure. In WT animals, DOCA-salt treatment increased the aortic medial area compared with the non-treated controls. Similarly, Tgfb1 was overexpressed in the aortas of the DOCA-salt treated WT mice compared with the controls. Immunofluorescence staining revealed that fibroblast-specific protein 1 (FSP1) + -α smooth muscle actin (αSMA) + myofibroblasts exist in the medial area of the WT aortas after DOCA-salt intervention. Importantly, these changes were not observed in the Klf5 Postn KO animals. In conclusion, the results of this study suggest that the presence of KLF5 in postn-positive cells contributes to the pathogenesis of aortic thickening induced by DOCA-salt hypertension.

Original languageEnglish (US)
Pages (from-to)764-768
Number of pages5
JournalHypertension Research
Volume39
Issue number11
DOIs
StatePublished - Nov 1 2016

Keywords

  • Krüppel-like factor 5
  • deoxycorticosterone-acetate salt
  • periostin
  • remodeling

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Zempo, H., Suzuki, J. I., Ogawa, M., Watanabe, R., Fujiu, K., Manabe, I., Conway, S. J., Taniyama, Y., Morishita, R., Hirata, Y., Isobe, M., & Nagai, R. (2016). Influence of periostin-positive cell-specific Klf5 deletion on aortic thickening in DOCA-salt hypertensive mice. Hypertension Research, 39(11), 764-768. https://doi.org/10.1038/hr.2016.65