Influence of periostin-positive cell-specific Klf5 deletion on aortic thickening in DOCA-salt hypertensive mice

Hirofumi Zempo, Jun Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Katsuhito Fujiu, Ichiro Manabe, Simon J. Conway, Yoshiaki Taniyama, Ryuichi Morishita, Yasunobu Hirata, Mitsuaki Isobe, Ryozo Nagai

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2 Scopus citations


Chronic hypertension causes vascular remodeling that is associated with an increase in periostin- (postn) positive cells, including fibroblasts and smooth muscle cells. Krüppel-like factor (KLF) 5, a transcription factor, is also observed in vascular remodeling; however, it is unknown what role KLF5 plays in postn-positive cells during vascular remodeling induced by deoxycorticosterone-acetate (DOCA) salt. We used postn-positive cell-specific Klf5-deficient mice (Klf5 Postn KO: Klf5 flox/flox; Postn Cre/-) and wild-type mice (WT: Klf5 flox/flox; Postn -/-). We implanted a DOCA pellet and provided drinking water containing 0.9% NaCl for 8 weeks. The DOCA-salt treatment induced hypertension in both genotypes, as observed by increases in systolic blood pressure. In WT animals, DOCA-salt treatment increased the aortic medial area compared with the non-treated controls. Similarly, Tgfb1 was overexpressed in the aortas of the DOCA-salt treated WT mice compared with the controls. Immunofluorescence staining revealed that fibroblast-specific protein 1 (FSP1) + -α smooth muscle actin (αSMA) + myofibroblasts exist in the medial area of the WT aortas after DOCA-salt intervention. Importantly, these changes were not observed in the Klf5 Postn KO animals. In conclusion, the results of this study suggest that the presence of KLF5 in postn-positive cells contributes to the pathogenesis of aortic thickening induced by DOCA-salt hypertension.

Original languageEnglish (US)
Pages (from-to)764-768
Number of pages5
JournalHypertension Research
Issue number11
StatePublished - Nov 1 2016



  • Krüppel-like factor 5
  • deoxycorticosterone-acetate salt
  • periostin
  • remodeling

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Zempo, H., Suzuki, J. I., Ogawa, M., Watanabe, R., Fujiu, K., Manabe, I., Conway, S. J., Taniyama, Y., Morishita, R., Hirata, Y., Isobe, M., & Nagai, R. (2016). Influence of periostin-positive cell-specific Klf5 deletion on aortic thickening in DOCA-salt hypertensive mice. Hypertension Research, 39(11), 764-768.