Influence of sex on gene expression in human corneal epithelial cells.

Tomo Suzuki, Stephen M. Richards, Shaohui Liu, Roderick V. Jensen, David A. Sullivan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

PURPOSE: Sex-associated differences have been identified in the anatomy, physiology and pathophysiology of the human cornea. We hypothesize that many of these differences are due to fundamental variations in gene expression. Our objective in this study was to determine whether such differences exist in human corneal epithelial cells both in vivo and in vitro. METHODS: Human corneal epithelial cells were isolated from the corneoscleral rims of male and female donors. Cells were processed either directly for RNA extraction, or first cultured in phenol red-free keratinocyte serum-free media. The RNA samples were examined for differentially expressed mRNAs by using of CodeLink Bioarrays and Affymetrix GeneChips. Data were analyzed with GeneSifter.Net software. RESULTS: Our results demonstrate that sex significantly influences the expression of over 600 genes in human corneal epithelial cells in vivo. These genes are involved in a broad spectrum of biologic processes, molecular functions and cellular components, such as metabolic processes, DNA replication, cell migration, RNA binding, oxidoreductase activity and nucleoli. We also identified significant, sex-related effects on gene expression in human corneal epithelial cells in vitro. However, with few exceptions (e.g., X- and Y-linked genes), these sex-related differences in gene expression in vitro were typically different than those in vivo. CONCLUSIONS: Our findings support our hypothesis that sex-related differences exist in the gene expression of human corneal epithelial cells. Variations in gene expression may contribute to sex-related differences in the prevalence of certain corneal diseases.

Original languageEnglish (US)
Pages (from-to)2554-2569
Number of pages16
JournalMolecular vision
Volume15
StatePublished - Jan 1 2009
Externally publishedYes

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Epithelial Cells
Gene Expression
Sex Characteristics
RNA
Y-Linked Genes
Corneal Diseases
Phenolsulfonphthalein
X-Linked Genes
Serum-Free Culture Media
DNA Replication
Keratinocytes
Cornea
Genes
Cell Movement
Anatomy
Oxidoreductases
Software
Messenger RNA
In Vitro Techniques

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Suzuki, T., Richards, S. M., Liu, S., Jensen, R. V., & Sullivan, D. A. (2009). Influence of sex on gene expression in human corneal epithelial cells. Molecular vision, 15, 2554-2569.

Influence of sex on gene expression in human corneal epithelial cells. / Suzuki, Tomo; Richards, Stephen M.; Liu, Shaohui; Jensen, Roderick V.; Sullivan, David A.

In: Molecular vision, Vol. 15, 01.01.2009, p. 2554-2569.

Research output: Contribution to journalArticle

Suzuki, T, Richards, SM, Liu, S, Jensen, RV & Sullivan, DA 2009, 'Influence of sex on gene expression in human corneal epithelial cells.', Molecular vision, vol. 15, pp. 2554-2569.
Suzuki T, Richards SM, Liu S, Jensen RV, Sullivan DA. Influence of sex on gene expression in human corneal epithelial cells. Molecular vision. 2009 Jan 1;15:2554-2569.
Suzuki, Tomo ; Richards, Stephen M. ; Liu, Shaohui ; Jensen, Roderick V. ; Sullivan, David A. / Influence of sex on gene expression in human corneal epithelial cells. In: Molecular vision. 2009 ; Vol. 15. pp. 2554-2569.
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