Influence of tamoxifen on ultrastructure and cell kinetics of 7,12-dimethylbenz[α]anthracene-induced mammary tumor cells in primary culture

M. T. Tseng, Ahmad Safa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An enzymatically dispersed, gradient enriched cell subpopulation of a 7,12-dimethylbenz[α]anthracene (DMBA)-induced mammary tumor was maintained in the primary culture for the purpose of evaluating therapeutic agents. The influence of tamoxifen on cell growth parameters and the ultrastructure of the surviving cells was examined. During a 12-hour treatment period, the surviving fraction ranged from 95%-50% in drug concentrations between 10-9-10-5 M. At 10-4 M all cells were killed. Thymidine incorporation was reduced in surviving cells in parallel with the changes in the other growth parameters investigated. Ultrastructural examination of the attached cells revealed a high degree of homogeneity and nearly all cells accumulated myelin bodies. Inasmuch as these cells appeared to be minimally influenced by tamoxifen they may represent the hormone independent portion of the DMBA-induced mammary tumor in rats.

Original languageEnglish (US)
Pages (from-to)281-282
Number of pages2
JournalIRCS Medical Science
Volume9
Issue number4
StatePublished - 1981
Externally publishedYes

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Primary Cell Culture
Tamoxifen
Cell culture
Tumors
Cells
Breast Neoplasms
Kinetics
Cell growth
Thymidine
Rats
Hormones
Pharmaceutical Preparations
anthracene
Growth
Myelin Sheath

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

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abstract = "An enzymatically dispersed, gradient enriched cell subpopulation of a 7,12-dimethylbenz[α]anthracene (DMBA)-induced mammary tumor was maintained in the primary culture for the purpose of evaluating therapeutic agents. The influence of tamoxifen on cell growth parameters and the ultrastructure of the surviving cells was examined. During a 12-hour treatment period, the surviving fraction ranged from 95{\%}-50{\%} in drug concentrations between 10-9-10-5 M. At 10-4 M all cells were killed. Thymidine incorporation was reduced in surviving cells in parallel with the changes in the other growth parameters investigated. Ultrastructural examination of the attached cells revealed a high degree of homogeneity and nearly all cells accumulated myelin bodies. Inasmuch as these cells appeared to be minimally influenced by tamoxifen they may represent the hormone independent portion of the DMBA-induced mammary tumor in rats.",
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