An enzymatically dispersed, gradient enriched cell subpopulation of a 7,12-dimethylbenz[α]anthracene (DMBA)-induced mammary tumor was maintained in the primary culture for the purpose of evaluating therapeutic agents. The influence of tamoxifen on cell growth parameters and the ultrastructure of the surviving cells was examined. During a 12-hour treatment period, the surviving fraction ranged from 95%-50% in drug concentrations between 10-9-10-5 M. At 10-4 M all cells were killed. Thymidine incorporation was reduced in surviving cells in parallel with the changes in the other growth parameters investigated. Ultrastructural examination of the attached cells revealed a high degree of homogeneity and nearly all cells accumulated myelin bodies. Inasmuch as these cells appeared to be minimally influenced by tamoxifen they may represent the hormone independent portion of the DMBA-induced mammary tumor in rats.
|Original language||English (US)|
|Number of pages||2|
|Journal||IRCS Medical Science|
|State||Published - Jan 1 1981|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)