Influence of treatment of diabetic rats with combinations of pycnogenol, β-carotene, and α-lipoic acid on parameters of oxidative stress

Abbie M. Berryman, A. C. Maritim, R. A. Sanders, J. B. Watkins

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Treatment with antioxidants may act more effectively to alter markers of free radical damage in combinations than singly. This study has determined whether treatment with combinations of pycnogenol, β-carotene, and α-lipoic acid was more effective at reducing oxidative stress in diabetic rats than treatment with these antioxidants alone. It is not feasible, based on this study, to assume that there are interactive effects that make combinations of these antioxidants more effective than any one alone to combat oxidative stress. Female Sprague-Dawley rats, normal and streptozotocin-induced diabetic, were treated (10 mg/ kg/day ip for 14 days) with pycnogenol, β-carotene, pycnogenol + β-carotene, or pycnogenol + β-carotene + α-lipoic acid; controls were untreated. Concentrations of thiobarbituric acid reactive substances, glutathione and glutathione disulfide, and activities of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase were measured in liver, kidney, and heart. Four types of effects were observed: (1) treatment with β-carotene alone either reversed (cardiac glutathione disulfide) or elevated (cardiac glutathione, hepatic glutathione peroxidase activity) levels seen in diabetic animals; (2) β-carotene alone produced no effect, but pycnogenol both alone and in combinations elevated (renal glutathione peroxidase and glutathione reductase activities, hepatic glutathione reductase activity and glutathione disulfide) or depressed (cardiac glutathione disulfide) levels seen in untreated diabetic animals; (3) all treatments with antioxidants, either alone or in combination, either normalized (lipid peroxidation in all tissues), elevated (hepatic GSH, cardiac glutathione peroxidase activity), or had no effect on (activities of hepatic catalase and superoxide dismutase in all tissues) levels seen in diabetic animals; (4) in only one case (cardiac glutathione reductase activity) levels in diabetic animals treated with combinations of antioxidants were normal, but elevated in animals treated with either antioxidant alone. Antioxidant effects seem to be dependent on the nature of the antioxidant used and not on combination effects.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalJournal of Biochemical and Molecular Toxicology
Volume18
Issue number6
DOIs
StatePublished - 2004

Fingerprint

Thioctic Acid
Oxidative stress
Carotenoids
Rats
Oxidative Stress
Antioxidants
Glutathione Disulfide
Glutathione Reductase
Glutathione Peroxidase
Animals
Liver
Catalase
Superoxide Dismutase
Glutathione
Tissue
Kidney
Thiobarbituric Acid Reactive Substances
pycnogenols
Streptozocin
Lipid Peroxidation

Keywords

  • Antioxidant
  • Diabetes
  • Oxidative Stress
  • Rat
  • Streptozotocin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Influence of treatment of diabetic rats with combinations of pycnogenol, β-carotene, and α-lipoic acid on parameters of oxidative stress. / Berryman, Abbie M.; Maritim, A. C.; Sanders, R. A.; Watkins, J. B.

In: Journal of Biochemical and Molecular Toxicology, Vol. 18, No. 6, 2004, p. 345-352.

Research output: Contribution to journalArticle

@article{26c7cb425ca548429c4b99d4548250c7,
title = "Influence of treatment of diabetic rats with combinations of pycnogenol, β-carotene, and α-lipoic acid on parameters of oxidative stress",
abstract = "Treatment with antioxidants may act more effectively to alter markers of free radical damage in combinations than singly. This study has determined whether treatment with combinations of pycnogenol, β-carotene, and α-lipoic acid was more effective at reducing oxidative stress in diabetic rats than treatment with these antioxidants alone. It is not feasible, based on this study, to assume that there are interactive effects that make combinations of these antioxidants more effective than any one alone to combat oxidative stress. Female Sprague-Dawley rats, normal and streptozotocin-induced diabetic, were treated (10 mg/ kg/day ip for 14 days) with pycnogenol, β-carotene, pycnogenol + β-carotene, or pycnogenol + β-carotene + α-lipoic acid; controls were untreated. Concentrations of thiobarbituric acid reactive substances, glutathione and glutathione disulfide, and activities of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase were measured in liver, kidney, and heart. Four types of effects were observed: (1) treatment with β-carotene alone either reversed (cardiac glutathione disulfide) or elevated (cardiac glutathione, hepatic glutathione peroxidase activity) levels seen in diabetic animals; (2) β-carotene alone produced no effect, but pycnogenol both alone and in combinations elevated (renal glutathione peroxidase and glutathione reductase activities, hepatic glutathione reductase activity and glutathione disulfide) or depressed (cardiac glutathione disulfide) levels seen in untreated diabetic animals; (3) all treatments with antioxidants, either alone or in combination, either normalized (lipid peroxidation in all tissues), elevated (hepatic GSH, cardiac glutathione peroxidase activity), or had no effect on (activities of hepatic catalase and superoxide dismutase in all tissues) levels seen in diabetic animals; (4) in only one case (cardiac glutathione reductase activity) levels in diabetic animals treated with combinations of antioxidants were normal, but elevated in animals treated with either antioxidant alone. Antioxidant effects seem to be dependent on the nature of the antioxidant used and not on combination effects.",
keywords = "Antioxidant, Diabetes, Oxidative Stress, Rat, Streptozotocin",
author = "Berryman, {Abbie M.} and Maritim, {A. C.} and Sanders, {R. A.} and Watkins, {J. B.}",
year = "2004",
doi = "10.1002/jbt.20046",
language = "English",
volume = "18",
pages = "345--352",
journal = "Journal of Biochemical and Molecular Toxicology",
issn = "1095-6670",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Influence of treatment of diabetic rats with combinations of pycnogenol, β-carotene, and α-lipoic acid on parameters of oxidative stress

AU - Berryman, Abbie M.

AU - Maritim, A. C.

AU - Sanders, R. A.

AU - Watkins, J. B.

PY - 2004

Y1 - 2004

N2 - Treatment with antioxidants may act more effectively to alter markers of free radical damage in combinations than singly. This study has determined whether treatment with combinations of pycnogenol, β-carotene, and α-lipoic acid was more effective at reducing oxidative stress in diabetic rats than treatment with these antioxidants alone. It is not feasible, based on this study, to assume that there are interactive effects that make combinations of these antioxidants more effective than any one alone to combat oxidative stress. Female Sprague-Dawley rats, normal and streptozotocin-induced diabetic, were treated (10 mg/ kg/day ip for 14 days) with pycnogenol, β-carotene, pycnogenol + β-carotene, or pycnogenol + β-carotene + α-lipoic acid; controls were untreated. Concentrations of thiobarbituric acid reactive substances, glutathione and glutathione disulfide, and activities of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase were measured in liver, kidney, and heart. Four types of effects were observed: (1) treatment with β-carotene alone either reversed (cardiac glutathione disulfide) or elevated (cardiac glutathione, hepatic glutathione peroxidase activity) levels seen in diabetic animals; (2) β-carotene alone produced no effect, but pycnogenol both alone and in combinations elevated (renal glutathione peroxidase and glutathione reductase activities, hepatic glutathione reductase activity and glutathione disulfide) or depressed (cardiac glutathione disulfide) levels seen in untreated diabetic animals; (3) all treatments with antioxidants, either alone or in combination, either normalized (lipid peroxidation in all tissues), elevated (hepatic GSH, cardiac glutathione peroxidase activity), or had no effect on (activities of hepatic catalase and superoxide dismutase in all tissues) levels seen in diabetic animals; (4) in only one case (cardiac glutathione reductase activity) levels in diabetic animals treated with combinations of antioxidants were normal, but elevated in animals treated with either antioxidant alone. Antioxidant effects seem to be dependent on the nature of the antioxidant used and not on combination effects.

AB - Treatment with antioxidants may act more effectively to alter markers of free radical damage in combinations than singly. This study has determined whether treatment with combinations of pycnogenol, β-carotene, and α-lipoic acid was more effective at reducing oxidative stress in diabetic rats than treatment with these antioxidants alone. It is not feasible, based on this study, to assume that there are interactive effects that make combinations of these antioxidants more effective than any one alone to combat oxidative stress. Female Sprague-Dawley rats, normal and streptozotocin-induced diabetic, were treated (10 mg/ kg/day ip for 14 days) with pycnogenol, β-carotene, pycnogenol + β-carotene, or pycnogenol + β-carotene + α-lipoic acid; controls were untreated. Concentrations of thiobarbituric acid reactive substances, glutathione and glutathione disulfide, and activities of glutathione reductase, glutathione peroxidase, superoxide dismutase, and catalase were measured in liver, kidney, and heart. Four types of effects were observed: (1) treatment with β-carotene alone either reversed (cardiac glutathione disulfide) or elevated (cardiac glutathione, hepatic glutathione peroxidase activity) levels seen in diabetic animals; (2) β-carotene alone produced no effect, but pycnogenol both alone and in combinations elevated (renal glutathione peroxidase and glutathione reductase activities, hepatic glutathione reductase activity and glutathione disulfide) or depressed (cardiac glutathione disulfide) levels seen in untreated diabetic animals; (3) all treatments with antioxidants, either alone or in combination, either normalized (lipid peroxidation in all tissues), elevated (hepatic GSH, cardiac glutathione peroxidase activity), or had no effect on (activities of hepatic catalase and superoxide dismutase in all tissues) levels seen in diabetic animals; (4) in only one case (cardiac glutathione reductase activity) levels in diabetic animals treated with combinations of antioxidants were normal, but elevated in animals treated with either antioxidant alone. Antioxidant effects seem to be dependent on the nature of the antioxidant used and not on combination effects.

KW - Antioxidant

KW - Diabetes

KW - Oxidative Stress

KW - Rat

KW - Streptozotocin

UR - http://www.scopus.com/inward/record.url?scp=12844269794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12844269794&partnerID=8YFLogxK

U2 - 10.1002/jbt.20046

DO - 10.1002/jbt.20046

M3 - Article

C2 - 15674846

AN - SCOPUS:12844269794

VL - 18

SP - 345

EP - 352

JO - Journal of Biochemical and Molecular Toxicology

JF - Journal of Biochemical and Molecular Toxicology

SN - 1095-6670

IS - 6

ER -