Inhibition of bacterial adhesion by antibacterial surface pretreatment of vascular prostheses

Lawrence X. Webb, Richard T. Myers, A. Robert Cordell, Cherri Hobgood, J. William Costerton, Anthony G. Gristina

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Polytetrafluoroethylene grafts were pretreated with oxacillin, with the cationic detergent benzalkonium, or with both substances, either at room temperature or at 90°C. Inhibition zones ranging from 6.4 to 15.2 mm formed around all grafts incubated on Staphylococcus aureus-streaked agar plates except control disks and those treated with oxacillin. Treated grafts were exposed in vitro to S. aureus in high concentration, followed by distilled water lavage. The graft surface was then stained with ruthenium red to stain polysaccharides and studied by scanning and transmission electron microscopy. Colonization of the graft surface by adhesive bacteria was demonstrated in all cases, although it was less prevalent on grafts pretreated with benzalkonium bound at 90°C.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalJournal of Vascular Surgery
Volume4
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Blood Vessel Prosthesis
Bacterial Adhesion
Transplants
Benzalkonium Compounds
Oxacillin
Staphylococcus aureus
Ruthenium Red
Scanning Transmission Electron Microscopy
Therapeutic Irrigation
Polytetrafluoroethylene
Adhesives
Detergents
Agar
Polysaccharides
Coloring Agents
Bacteria
Temperature
Water

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine

Cite this

Inhibition of bacterial adhesion by antibacterial surface pretreatment of vascular prostheses. / Webb, Lawrence X.; Myers, Richard T.; Cordell, A. Robert; Hobgood, Cherri; Costerton, J. William; Gristina, Anthony G.

In: Journal of Vascular Surgery, Vol. 4, No. 1, 1986, p. 16-21.

Research output: Contribution to journalArticle

Webb, Lawrence X. ; Myers, Richard T. ; Cordell, A. Robert ; Hobgood, Cherri ; Costerton, J. William ; Gristina, Anthony G. / Inhibition of bacterial adhesion by antibacterial surface pretreatment of vascular prostheses. In: Journal of Vascular Surgery. 1986 ; Vol. 4, No. 1. pp. 16-21.
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