Inhibition of DNA replication by ultraviolet light

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Abstract

Ultraviolet irradiation is known to inhibit DNA synthesis in mammalian cells, although the mechanism(s) of this inhibition is (are) not known. In this paper DNA replication in u.v. irradiated HeLa cells was studied by 2 different techniques: measurements of the kinetics of semiconservative DNA synthesis and DNA fiber autoradiography. In examining the kinetics of semiconservative DNA synthesis, density label was used to avoid measuring the incorporation due to repair replication. The extent of inhibition varied with time. After doses of less than 10 J/m2 the rate was initially depressed but later showed some recovery. After higher doses, a constant, low rate of synthesis was seen for at least the initial 6 h. An analysis of these data indicated that the inhibition of DNA synthesis could be explained by replication forks halting at pyrimidine dimers. DNA fiber autoradiography was used to further characterize replication after ultraviolet irradiation. The average length of labeled segments in irradiated cells increased in the time immediately after irradiation, and then leveled off. This is the predicted pattern if DNA synthesis in each replicon continued at its previous rate until a lesion is reached, and then halted. The frequency of lesions that block synthesis is approximately the same as the frequency of pyrimidine dimers.

Original languageEnglish (US)
Pages (from-to)849-860
Number of pages12
JournalBiophysical Journal
Volume16
Issue number8
StatePublished - 1976
Externally publishedYes

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Ultraviolet Rays
DNA Replication
DNA
Pyrimidine Dimers
Autoradiography
Replicon
HeLa Cells

ASJC Scopus subject areas

  • Biophysics

Cite this

Inhibition of DNA replication by ultraviolet light. / Edenberg, Howard.

In: Biophysical Journal, Vol. 16, No. 8, 1976, p. 849-860.

Research output: Contribution to journalArticle

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