Inhibition of glycolipid shedding rescues recognition of a CD1+ T cell lymphoma by natural killer T (NKT) cells

Venkataraman Sriram, Sungyoo Cho, Ping Li, Patrick W. O'Donnell, Claire Dunn, Kyoko Hayakawa, Janice Blum, Randy Brutkiewicz

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Abstract

Neoplastic transformation of cells is accompanied by an aberration of cell surface glycolipid composition. These tumor-associated, altered glycosphingolipids are often shed into the tumor cell microenvironment and mediate immunosuppressive activity. The nature and form of glycolipids shed by a variety of tumor cell lines and the mechanism(s) of shedding have been well characterized. The murine T cell lymphoma line, L5178Y-R, is known to shed a tumor-associated glycolipid, gangliotriaosylceramide, into the culture medium. We analyzed the effect of glycolipids from L5178Y-R on antigen presentation by murine CD1d1 molecules. CD1d1 molecules present glycolipid antigens to a specialized class of T cells called natural killer T (NKT) cells that mainly express a T cell receptor α chain (Vα14Jα281) associated with Vβ chains of limited diversity. In the current report, we found that L5178Y-R cells express CD1 on their cell surface yet are unable to stimulate CD1d1-specific NKT cells. We hypothesized that the glycolipid(s) shed by L5178Y-R inhibited antigen presentation by CD1d1. Pretreatment of CD1d1+ cells with conditioned medium from L5178Y-R inhibited CD1-specific stimulation of canonical (Vα14+) but not noncanonical (Vα5+) NKT cells. Exogenous addition of lipids extracted from L5178Y-R cells as well as purified gangliotriaosylceramide mimicked this effect. Inhibition of glycolipid shedding in L5178Y-R cells with D-1-phenyl-2-hexadecanoylamino-3-morpholino-1-propanol resulted in the rescue of CD1d1 recognition by canonical (but not noncanonical) NKT cells. These results suggest that one means by which certain tumor cells can evade the host's innate antitumor immune response is by shedding glycolipids that inhibit CD1-mediated antigen presentation to NKT cells.

Original languageEnglish
Pages (from-to)8197-8202
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number12
DOIs
StatePublished - Jun 11 2002

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Natural Killer T-Cells
T-Cell Lymphoma
Glycolipids
Antigen Presentation
CD1 Antigens
Neoplastic Cell Transformation
Cellular Microenvironment
Neoplasms
Glycosphingolipids
Tumor Microenvironment
Immunosuppressive Agents
Conditioned Culture Medium
T-Cell Antigen Receptor
Tumor Cell Line
Innate Immunity
Culture Media
T-Lymphocytes
Lipids
Antigens
Cell Line

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Inhibition of glycolipid shedding rescues recognition of a CD1+ T cell lymphoma by natural killer T (NKT) cells. / Sriram, Venkataraman; Cho, Sungyoo; Li, Ping; O'Donnell, Patrick W.; Dunn, Claire; Hayakawa, Kyoko; Blum, Janice; Brutkiewicz, Randy.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 12, 11.06.2002, p. 8197-8202.

Research output: Contribution to journalArticle

Sriram, Venkataraman ; Cho, Sungyoo ; Li, Ping ; O'Donnell, Patrick W. ; Dunn, Claire ; Hayakawa, Kyoko ; Blum, Janice ; Brutkiewicz, Randy. / Inhibition of glycolipid shedding rescues recognition of a CD1+ T cell lymphoma by natural killer T (NKT) cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 12. pp. 8197-8202.
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