Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera

R. J. Winchester, P. A. Meyers, Hal Broxmeyer, C. Y. Wang, M. A. Moore, H. G. Kunkel

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Incubation with Ia antiserum, followed by complement, markedly inhibited erythroid colonies arising from hematopoietic cells present in the nonadherent low density fractions of normal bone marrow. Both erythropoietin-dependent colonies and bursts were eliminated at dilutions of antiserum equivalent to, or greater than, the dilutions required to abolish the granulocyte-macrophage colony formation. The inhibitory effect of the Ia antiserum was abolished by absorption with B but not T cells from lymphoid lines. Available evidence suggested that Ia determinants are expressed on the erythropoietin-sensitive progenitors of the erythroid series in precise analogy to their sequence of expression on the granulocyte lineage. In both lineages, as shown previously, the Ia determinants become undetectable during subsequent stages of differentiation.

Original languageEnglish (US)
Pages (from-to)613-618
Number of pages6
JournalJournal of Experimental Medicine
Volume148
Issue number2
StatePublished - 1978
Externally publishedYes

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Immune Sera
Erythropoietin
Granulocytes
Bone Marrow
Macrophages
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Winchester, R. J., Meyers, P. A., Broxmeyer, H., Wang, C. Y., Moore, M. A., & Kunkel, H. G. (1978). Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera. Journal of Experimental Medicine, 148(2), 613-618.

Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera. / Winchester, R. J.; Meyers, P. A.; Broxmeyer, Hal; Wang, C. Y.; Moore, M. A.; Kunkel, H. G.

In: Journal of Experimental Medicine, Vol. 148, No. 2, 1978, p. 613-618.

Research output: Contribution to journalArticle

Winchester, RJ, Meyers, PA, Broxmeyer, H, Wang, CY, Moore, MA & Kunkel, HG 1978, 'Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera', Journal of Experimental Medicine, vol. 148, no. 2, pp. 613-618.
Winchester, R. J. ; Meyers, P. A. ; Broxmeyer, Hal ; Wang, C. Y. ; Moore, M. A. ; Kunkel, H. G. / Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera. In: Journal of Experimental Medicine. 1978 ; Vol. 148, No. 2. pp. 613-618.
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