Inhibition of human renal epithelial Na+/Pi cotransport by phosphonoformic acid

Ahad N.K. Yusufi, Miroslawa Szczepanska-Konkel, Stephen A. Kempson, James A. McAteer, Thomas P. Dousa

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Phosphonoformic acid was a potent competitive inhibitor of Na+/Pi cotransport by brush border membrane vesicles isolated from human renal cortex (Ki 31 uM). The Na+/Pi cotransport system in rat renal brush border membrane vesicles was much less sensitive to inhibition (Ki 210 uM). Na+/Pi cotransport by cultured cells derived from human renal cortex also was inhibited by phosphonoformic acid. Both in isolated membranes and cells the inhibition was dose-dependent, competitive, and specific for Pi. The effectiveness of phosphonoformic acid on Na+/Pi cotransport in human kidney suggests that its administration may be a useful maneuver to increase renal Pi excretion.

Original languageEnglish (US)
Pages (from-to)679-686
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number2
DOIs
StatePublished - Sep 16 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Yusufi, A. N. K., Szczepanska-Konkel, M., Kempson, S. A., McAteer, J. A., & Dousa, T. P. (1986). Inhibition of human renal epithelial Na+/Pi cotransport by phosphonoformic acid. Biochemical and Biophysical Research Communications, 139(2), 679-686. https://doi.org/10.1016/S0006-291X(86)80044-4