Phosphonoformic acid was a potent competitive inhibitor of Na+/Pi cotransport by brush border membrane vesicles isolated from human renal cortex (Ki 31 uM). The Na+/Pi cotransport system in rat renal brush border membrane vesicles was much less sensitive to inhibition (Ki 210 uM). Na+/Pi cotransport by cultured cells derived from human renal cortex also was inhibited by phosphonoformic acid. Both in isolated membranes and cells the inhibition was dose-dependent, competitive, and specific for Pi. The effectiveness of phosphonoformic acid on Na+/Pi cotransport in human kidney suggests that its administration may be a useful maneuver to increase renal Pi excretion.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 16 1986|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology