Inhibition of microvascular endothelial cell proliferation by vitreous following retinal scatter photocoagulation

Arvind Singh, Mike Boulton, Carol Lane, John Forrester, Joe Gaal, David McLeod

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Laser photocoagulation of pig retina induced breakdown of the blood-retinal barrier, with the appearance of serum proteins in the vitreous as determined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting techniques. Vitreous from lasered eyes inhibited the proliferation of cultured retinal microvascular endothelial cells in comparison with vitreous from non-lasered control eyes, and the inhibitory effect in the lasered eyes persisted for at least seven days. Inhibition was specific for endothelial cells, since no effect was observed when retinal pericytes or Tenon's fibroblasts were the target cells. These results suggest that indirect scatter photocoagulation may induce regression of neovascularisation by causing breakdown of the blood-retinal barrier and thus releasing into the vitreous serum components which result in inhibition of retinal microvascular endothelial cell growth.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalBritish Journal of Ophthalmology
Volume74
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

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Light Coagulation
Blood-Retinal Barrier
Endothelial Cells
Cell Proliferation
Pericytes
Immunoblotting
Sodium Dodecyl Sulfate
Retina
Blood Proteins
Polyacrylamide Gel Electrophoresis
Lasers
Swine
Fibroblasts
Growth
Serum

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Ophthalmology

Cite this

Inhibition of microvascular endothelial cell proliferation by vitreous following retinal scatter photocoagulation. / Singh, Arvind; Boulton, Mike; Lane, Carol; Forrester, John; Gaal, Joe; McLeod, David.

In: British Journal of Ophthalmology, Vol. 74, No. 6, 1990, p. 328-332.

Research output: Contribution to journalArticle

Singh, Arvind ; Boulton, Mike ; Lane, Carol ; Forrester, John ; Gaal, Joe ; McLeod, David. / Inhibition of microvascular endothelial cell proliferation by vitreous following retinal scatter photocoagulation. In: British Journal of Ophthalmology. 1990 ; Vol. 74, No. 6. pp. 328-332.
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