Inhibition of protein synthesis and cell proliferation in cultured human breast cancer cells treated with mitoxantrone

Ahmad Safa, Michael T. Tseng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mitoxantrone suppresses cell proliferation, inhibits protein synthesis and induces ultrastructural alterations in the T-47D and MDA-MB-231 breast cancer cell lines. After 24 h treatment with 10-9, 10-7 and 10-5 M drug and 8 h incubation with [35S]methionine, protein synthesis declined rapidly. While a 10-15% decrease in protein synthesis at 10-9 M was observed, more than 95% inhibition of protein synthesis occurred at 10-5 M mitoxantrone in both cell lines. Sodium dodecylsulfate (SDS) gel electrophoresis of labeled proteins revealed no qualitative changes in either cell line. However, only trace amounts of several proteins were present in T-47D cells treated with 10-5 M drug. At 10-9 M mitoxantrone had little effect on cell proliferation. At 10-7 M, 25% and 35% growth inhibition in T-47D and MDA-MB-231 cells was observed, respectively. Cell growth at 10-5 M was abolished. Cytotoxicity was evident at drug concentrations above 10-5 M. Ultrastructural alterations in the nucleoli of both cell lines included disintegration and segregation of granular and fibrillar components and the disappearance of nucleolar organizers at 10-7-10-5 M mitoxantrone.

Original languageEnglish (US)
Pages (from-to)317-326
Number of pages10
JournalCancer Letters
Volume24
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Mitoxantrone
Cell Proliferation
Breast Neoplasms
Cell Line
Proteins
Pharmaceutical Preparations
Nucleolus Organizer Region
Growth
Methionine
Electrophoresis
Gels
Sodium

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Inhibition of protein synthesis and cell proliferation in cultured human breast cancer cells treated with mitoxantrone. / Safa, Ahmad; Tseng, Michael T.

In: Cancer Letters, Vol. 24, No. 3, 1984, p. 317-326.

Research output: Contribution to journalArticle

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abstract = "Mitoxantrone suppresses cell proliferation, inhibits protein synthesis and induces ultrastructural alterations in the T-47D and MDA-MB-231 breast cancer cell lines. After 24 h treatment with 10-9, 10-7 and 10-5 M drug and 8 h incubation with [35S]methionine, protein synthesis declined rapidly. While a 10-15{\%} decrease in protein synthesis at 10-9 M was observed, more than 95{\%} inhibition of protein synthesis occurred at 10-5 M mitoxantrone in both cell lines. Sodium dodecylsulfate (SDS) gel electrophoresis of labeled proteins revealed no qualitative changes in either cell line. However, only trace amounts of several proteins were present in T-47D cells treated with 10-5 M drug. At 10-9 M mitoxantrone had little effect on cell proliferation. At 10-7 M, 25{\%} and 35{\%} growth inhibition in T-47D and MDA-MB-231 cells was observed, respectively. Cell growth at 10-5 M was abolished. Cytotoxicity was evident at drug concentrations above 10-5 M. Ultrastructural alterations in the nucleoli of both cell lines included disintegration and segregation of granular and fibrillar components and the disappearance of nucleolar organizers at 10-7-10-5 M mitoxantrone.",
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