Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin

Ming Xian, Kun Wang, Xinchao Chen, Yongchun Hou, Andrea McGill, Xi Chen, Bo Zhou, Zhong Yin Zhang, Jin Pei Cheng, Peng George Wang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order rate constants (k(inact)/K(I)) ranging from 37 to 113 M-1 min-1 against mammalian PTP1B and from 66 to 613 M-1 min-1 against Yersinia PTPase. Furthermore, the inactivation of Yersinia PTPase by S-nitrosylated protein:S-nitroso human serum albumin was investigated. Both single-S-nitrosylated and poly-S-nitrosylated human serum albumin show good inhibitory ability to Yersinia PTPase. The second-order rate constants are 472 and 1188 M-1 min-1, respectively. This result indicates a possibility that S-nitrosylated albumin in vivo may function as an inhibitor for a variety of cysteine-dependent enzymes. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)310-314
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume268
Issue number2
DOIs
StatePublished - Feb 16 2000

Fingerprint

S-Nitrosothiols
Yersinia
Protein Tyrosine Phosphatases
Serum Albumin
Molecular Weight
Molecular weight
Non-Receptor Type 1 Protein Tyrosine Phosphatase
Rate constants
Recombinant proteins
Protein S
Recombinant Proteins
Cysteine
Albumins
Enzymes

Keywords

  • Inhibition
  • Nitric oxide
  • Protein tyrosine phosphatases
  • S-nitrosothiols
  • S-nitrosylated human serum albumin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin. / Xian, Ming; Wang, Kun; Chen, Xinchao; Hou, Yongchun; McGill, Andrea; Chen, Xi; Zhou, Bo; Zhang, Zhong Yin; Cheng, Jin Pei; Wang, Peng George.

In: Biochemical and Biophysical Research Communications, Vol. 268, No. 2, 16.02.2000, p. 310-314.

Research output: Contribution to journalArticle

Xian, Ming ; Wang, Kun ; Chen, Xinchao ; Hou, Yongchun ; McGill, Andrea ; Chen, Xi ; Zhou, Bo ; Zhang, Zhong Yin ; Cheng, Jin Pei ; Wang, Peng George. / Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 268, No. 2. pp. 310-314.
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