Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A

Gary M. Bokoch, Lawrence Quilliam, Benjamin P. Bohl, Algirdas J. Jesaitis, Mark T. Quinn

Research output: Contribution to journalArticle

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Abstract

Rap1A is a low molecular weight guanosine triphosphate (GTP)-binding protein in human neutrophil membranes whose cellular function is unknown. Rap1A was found to form stoichiometric complexes with the cytochrome b558 component of the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system. The (guanosine-5′-O-(3-thiotriphosphate) (GTP-γ-S)-bound form of Rap1A bound more tightly to cytochrome b558 than did the guanosine diphosphate-bound form. No complex formation was observed between cytochrome b558 and H-Ras-GTP-γ-S or Rap1A-GTP-γ-S that had been heat-inactivated, nor between Rap1A-GTP-γ-S and hydrophobic proteins serving as controls. Complex formation between Rap1A-GTP-γ-S and cytochrome b558 was inhibited by phosphorylation of Rap1A with cyclic adenosine monophosphate (cAMP)-dependent protein kinase. These observations suggest that Rap1A may participate in the structure or regulation of the NADPH oxidase system and that this function of the Rap1A protein may be altered by phosphorylation.

Original languageEnglish (US)
Pages (from-to)1794-1796
Number of pages3
JournalScience
Volume254
Issue number5039
StatePublished - Dec 20 1991
Externally publishedYes

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Guanosine Triphosphate
NADP
Oxidoreductases
Phosphorylation
rap1 GTP-Binding Proteins
Guanosine 5'-O-(3-Thiotriphosphate)
Guanosine
Diphosphates
Protein S
Phagocytes
Cyclic AMP-Dependent Protein Kinases
cytochrome b558
Carrier Proteins
Neutrophils
Hot Temperature
Molecular Weight
Membranes

ASJC Scopus subject areas

  • General

Cite this

Bokoch, G. M., Quilliam, L., Bohl, B. P., Jesaitis, A. J., & Quinn, M. T. (1991). Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A. Science, 254(5039), 1794-1796.

Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A. / Bokoch, Gary M.; Quilliam, Lawrence; Bohl, Benjamin P.; Jesaitis, Algirdas J.; Quinn, Mark T.

In: Science, Vol. 254, No. 5039, 20.12.1991, p. 1794-1796.

Research output: Contribution to journalArticle

Bokoch, GM, Quilliam, L, Bohl, BP, Jesaitis, AJ & Quinn, MT 1991, 'Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A', Science, vol. 254, no. 5039, pp. 1794-1796.
Bokoch GM, Quilliam L, Bohl BP, Jesaitis AJ, Quinn MT. Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A. Science. 1991 Dec 20;254(5039):1794-1796.
Bokoch, Gary M. ; Quilliam, Lawrence ; Bohl, Benjamin P. ; Jesaitis, Algirdas J. ; Quinn, Mark T. / Inhibition of Rap1A binding to cytochrome b558 of NADPH oxidase by phosphorylation of Rap1A. In: Science. 1991 ; Vol. 254, No. 5039. pp. 1794-1796.
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