Inhibition of the Accelerative Effects of Testosterone on Hamster Facial Nerve Regeneration by the Antiandrogen Flutamide

Kathleen A. Kujawa, Lisa Tanzer, Kathryn Jones

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have previously demonstrated that systemic administration of testosterone propionate (TP) to adult hamsters accelerates the rate of facial nerve regeneration following crush axotomy of the facial nerve at its exit from the stylomastoid foramen. In this study, we utilized flutamide, a potent nonsteroidal antiandrogen, in conjunction with radioisotopic labeling procedures for the assessment of facial nerve regeneration rates to test the hypothesis that TP exerts its accelerative effects on facial nerve regeneration through a receptor-mediated mechanism. Castrated adult male hamsters were subjected to right facial nerve crush axotomies and divided into three groups of axotomized animals: castrate plus one subcutaneous TP implant plus daily injections of flutamide, castrate plus one subcutaneous TP implant plus vehicle injections, and castrate only plus sham implant and vehicle injections. There were two postoperative timepoints: 4 and 7 days. In agreement with previous studies, systemic administration of TP resulted in an approximately 26% increase in the rate of regeneration of the fastest growing population of axons. Exposure to flutamide completely abolished the TP-induced accelerative effects on facial nerve regeneration rate. As a bioassay for the effectiveness of systemic administration of flutamide by subcutaneous injection, seminal vesicle weights were collected from all groups at the end of the postoperative time and compared as a percentage of the seminal vesicle weights of intact (nongonadectomized) male control animals. Castration greatly reduced seminal vesicle weights, whereas exogenous TP restored the seminal vesicle weights to those of the intact male. Flutamide blocked the effects of exogenous TP on seminal vesicle weights and reduced them to castrate levels. Thus, the results indicate that the mechanism by which gonadal steroids act to alter the regenerative properties of peripheral neurons is receptor-mediated.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalExperimental Neurology
Volume133
Issue number2
DOIs
StatePublished - Jun 1995
Externally publishedYes

Fingerprint

Flutamide
Testosterone Propionate
Androgen Antagonists
Nerve Regeneration
Facial Nerve
Cricetinae
Testosterone
Seminal Vesicles
Weights and Measures
Axotomy
Injections
Nonsteroidal Anti-Androgens
Nerve Crush
Castration
Subcutaneous Injections
Biological Assay
Axons
Regeneration
Steroids
Neurons

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Inhibition of the Accelerative Effects of Testosterone on Hamster Facial Nerve Regeneration by the Antiandrogen Flutamide. / Kujawa, Kathleen A.; Tanzer, Lisa; Jones, Kathryn.

In: Experimental Neurology, Vol. 133, No. 2, 06.1995, p. 138-143.

Research output: Contribution to journalArticle

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