Inhibitor of DNA binding 2 promotes sensory axonal growth after SCI

Panpan Yu, Yi Ping Zhang, Lisa B E Shields, Yiyan Zheng, Xiaoling Hu, Rachel Hill, Russell Howard, Zhen Gu, Darlene A. Burke, Scott R. Whittemore, Xiao-Ming Xu, Christopher B. Shields

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study investigated whether neuronal inhibitor of DNA binding 2 (Id2), a regulator of basic helix-loop-helix (bHLH) transcription factors, can activate the intrinsic neuritogenetic mode of dorsal root ganglion (DRG) neurons in adult mice following spinal cord injury (SCI).First, the Id2 developmental expression profile of DRG neurons, along with the correlated activity of Cdh1-anaphase promoting complex (Cdh1-APC), was characterized. Next, a D-box mutant Id2 (Id2DBM) adenoviral vector, resistant to Cdh1-APC degradation, was developed to enhance neuronal Id2 expression. After the vector was introduced into DRG neurons, the effect of Id2 on neurite outgrowth of cultured DRG neurons and sensory axonal regeneration following spinal cord dorsal hemisection was evaluated.The expression of Id2 in DRG neurons was high in the embryonic stage, downregulated after birth, and significantly reduced in the adult. Expression of Cdh1-APC was opposite to Id2, which may be responsible for Id2 degradation during DRG maturation. Overexpression of Id2DBM in DRG neurons enhanced neuritogenesis on both permissive and inhibitory substrates. Following spinal cord dorsal hemisection, overexpression of Id2DBM reduced axon dieback and increased the number and length of regenerative fibers into the lesion gap.Reprogramming the intrinsic growth status of quiescent adult DRG neurons by enhancing Id2 expression results in active neuritogenesis following SCI. Id2 may be a novel target for enhancing sensory axonal regeneration following injuries to the adult spinal cord.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalExperimental Neurology
Volume231
Issue number1
DOIs
StatePublished - Sep 2011

Fingerprint

Spinal Cord Injuries
Spinal Ganglia
DNA
Growth
Anaphase-Promoting Complex-Cyclosome
Neurons
Spinal Cord
Spinal Cord Regeneration
Basic Helix-Loop-Helix Transcription Factors
Sensory Receptor Cells
Axons
Regeneration
Down-Regulation
Parturition
Wounds and Injuries

Keywords

  • Axonal regeneration
  • DRG neuron
  • Gene delivery
  • Id2
  • Spinal cord injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Yu, P., Zhang, Y. P., Shields, L. B. E., Zheng, Y., Hu, X., Hill, R., ... Shields, C. B. (2011). Inhibitor of DNA binding 2 promotes sensory axonal growth after SCI. Experimental Neurology, 231(1), 38-44. https://doi.org/10.1016/j.expneurol.2011.05.013

Inhibitor of DNA binding 2 promotes sensory axonal growth after SCI. / Yu, Panpan; Zhang, Yi Ping; Shields, Lisa B E; Zheng, Yiyan; Hu, Xiaoling; Hill, Rachel; Howard, Russell; Gu, Zhen; Burke, Darlene A.; Whittemore, Scott R.; Xu, Xiao-Ming; Shields, Christopher B.

In: Experimental Neurology, Vol. 231, No. 1, 09.2011, p. 38-44.

Research output: Contribution to journalArticle

Yu, P, Zhang, YP, Shields, LBE, Zheng, Y, Hu, X, Hill, R, Howard, R, Gu, Z, Burke, DA, Whittemore, SR, Xu, X-M & Shields, CB 2011, 'Inhibitor of DNA binding 2 promotes sensory axonal growth after SCI', Experimental Neurology, vol. 231, no. 1, pp. 38-44. https://doi.org/10.1016/j.expneurol.2011.05.013
Yu, Panpan ; Zhang, Yi Ping ; Shields, Lisa B E ; Zheng, Yiyan ; Hu, Xiaoling ; Hill, Rachel ; Howard, Russell ; Gu, Zhen ; Burke, Darlene A. ; Whittemore, Scott R. ; Xu, Xiao-Ming ; Shields, Christopher B. / Inhibitor of DNA binding 2 promotes sensory axonal growth after SCI. In: Experimental Neurology. 2011 ; Vol. 231, No. 1. pp. 38-44.
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