Inhibitors of the ATP/ADP antiporter suppress stimulation of mitochondrial respiration and H+ permeability by palmitate and anionic detergents

N. N. Brustovetsky, V. I. Dedukhova, M. V. Egorova, E. N. Mokhova, V. P. Skulachev

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The action of ATP/ADP-antiporter inhibitors upon the uncoupling effect of palmitate, detergents and 'classical' uncouplers has been studied. The uncoupling effect was estimated by stimulation of succinate oxidation and of H+ permeability of rat liver mitochondria in the presence of oligomycin. It is shown that carboxyatractylate (CAtr) and pyridoxal 5-phosphate (PLP) suppress the uncoupling induced by palmitate and the anionic detergents SDS and cholate, but do not affect that induced by the cationic detergents CTAB, by the non-ionic detergent Triton X-100, as well as by the 'classical' uncouplers FCCP and DNP. The results are discussed in terms of a concept assuming that the ATP/ADP-antiporter facilitates the electrophoretic export of hydrophobic anions from mitochondria.

Original languageEnglish (US)
Pages (from-to)187-189
Number of pages3
JournalFEBS Letters
Volume272
Issue number1-2
DOIs
StatePublished - Oct 15 1990

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Keywords

  • ATP/ADP-antiporter
  • Fatty acid
  • Uncoupling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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