Inhibitors of the growth and aggregation of calcium oxalate crystals in vitro

W. G. Robertson, Munro Peacock, B. E C Nordin

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

A sensitive method has been developed for measuring the effect of inhibitors of crystallisation on the growth and aggregation of calcium oxalate crystals in vitro. Of the known inhibitors tested the highly charged anions pyrophosphate and ethane-1-hydroxy-1,1-diphosphonate (EHDP) were effective down to concentrations of 0.1-1 μmole/l and the polyanions heparin and chondroitin sulphate A down to concentrations of 1 nmole/l. Within the normal range of urea concentrations found in urine the neutral molecule urea caused a small but significant degree of inhibition. At higher concentrations the degree of inhibition increased sharply. In the concentrations tested the cations magnesium and methylene blue had no effect on the growth and aggregation of crystals. These findings suggest that highly charged anions and polyanions may have some clinical value as a possible treatment for recurrent calcium oxalate stone-formation in man.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalClinica Chimica Acta
Volume43
Issue number1
DOIs
StatePublished - Jan 10 1973
Externally publishedYes

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Growth Inhibitors
Calcium Oxalate
Crystallization
Anions
Urea
Agglomeration
Crystals
Ethane
Chondroitin Sulfates
Methylene Blue
Diphosphonates
Magnesium
Heparin
Cations
Reference Values
Urine
Molecules
Growth
polyanions
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Inhibitors of the growth and aggregation of calcium oxalate crystals in vitro. / Robertson, W. G.; Peacock, Munro; Nordin, B. E C.

In: Clinica Chimica Acta, Vol. 43, No. 1, 10.01.1973, p. 31-37.

Research output: Contribution to journalArticle

Robertson, W. G. ; Peacock, Munro ; Nordin, B. E C. / Inhibitors of the growth and aggregation of calcium oxalate crystals in vitro. In: Clinica Chimica Acta. 1973 ; Vol. 43, No. 1. pp. 31-37.
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