Inhibitory activity of l-asparaginase from Mycobcacterium tuberculosis on Yoshida ascites sarcoma in rats

V. V Subba Reddy, H. N. Jayaram, M. Sirsi, T. Ramakrishnan

Research output: Contribution to journalArticle

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Abstract

The antitumor activity of l-asparagine amidohydrolases (EC 3.5.1.1) from Mycobacterium tuberculosis H37Rv and H37Ra strains has been tested on Yoshida ascites sarcoma in rats. The enzyme specific to M. tuberculosis H37Ra but not to H37Rv has proved to be effective in inhibiting the growth of the sarcoma. Comparative studies on the activity of this enzyme with that of similar enzyme from Escherichia coli B, has shown that at the same levels the former is more effective than the latter. Long-lived immunity to this tumor in A/IISc Wistar rats following treatment of tumor bearing animals with M. tuberculosis H37Ra, pH 9.6 l-asparaginase has been observed. Immunity in these rats was demonstrated by tumor rejection and detection of humoral antibodies in the sera to the antigen of the cell-free extract of the tumor. The enzyme was ineffective in inhibiting fibrosarcoma in mice at the dose levels tested.

Original languageEnglish (US)
Pages (from-to)262-267
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume132
Issue number1
StatePublished - Jun 1969
Externally publishedYes

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Yoshida Sarcoma
Asparaginase
Ascites
Rats
Tumors
Tuberculosis
Mycobacterium tuberculosis
Enzymes
Immunity
Neoplasms
Bearings (structural)
Amidohydrolases
Fibrosarcoma
Asparagine
Cell Extracts
Sarcoma
Escherichia coli
Wistar Rats
Animals
Antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Inhibitory activity of l-asparaginase from Mycobcacterium tuberculosis on Yoshida ascites sarcoma in rats. / Reddy, V. V Subba; Jayaram, H. N.; Sirsi, M.; Ramakrishnan, T.

In: Archives of Biochemistry and Biophysics, Vol. 132, No. 1, 06.1969, p. 262-267.

Research output: Contribution to journalArticle

Reddy, V. V Subba ; Jayaram, H. N. ; Sirsi, M. ; Ramakrishnan, T. / Inhibitory activity of l-asparaginase from Mycobcacterium tuberculosis on Yoshida ascites sarcoma in rats. In: Archives of Biochemistry and Biophysics. 1969 ; Vol. 132, No. 1. pp. 262-267.
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