Mesenchymal cells from the wing buds of stage 24 chick embryos undergo differentiation to cartilage when plated at high density. Treatment of these cultures with phospholipase D resulted in inhibition of chondrogenesis. Phospholipase D treatment (which produces phosphatidic acid from membrane phospholipids) was found to affect cell proliferation and to dramatically increase intracellular free calcium levels and inositol phosphate production. Intracellular free Ca2+, mobilized as a result of phosphatidylinositol phosphate hydrolysis, may therefore inhibit chondrogenesis in embryonic mesenchymal cells.
ASJC Scopus subject areas
- Cell Biology
Inhibitory effects of phospholipase D on chondrogenesis in vitro. / Tsonis, Panagiotis A.; Del Rio-Tsonis, Katia; Rothrock, James; Dominguez, Jesus; English, Denis; Glade, Kimberly; Goetinck, Paul F.In: Experimental Cell Research, Vol. 195, No. 2, 08.1991, p. 551-555.
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