Inhibitory effects of phospholipase D on chondrogenesis in vitro

Panagiotis A. Tsonis, Katia Del Rio-Tsonis, James Rothrock, Jesus Dominguez, Denis English, Kimberly Glade, Paul F. Goetinck

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Mesenchymal cells from the wing buds of stage 24 chick embryos undergo differentiation to cartilage when plated at high density. Treatment of these cultures with phospholipase D resulted in inhibition of chondrogenesis. Phospholipase D treatment (which produces phosphatidic acid from membrane phospholipids) was found to affect cell proliferation and to dramatically increase intracellular free calcium levels and inositol phosphate production. Intracellular free Ca2+, mobilized as a result of phosphatidylinositol phosphate hydrolysis, may therefore inhibit chondrogenesis in embryonic mesenchymal cells.

Original languageEnglish (US)
Pages (from-to)551-555
Number of pages5
JournalExperimental Cell Research
Volume195
Issue number2
DOIs
StatePublished - Aug 1991

ASJC Scopus subject areas

  • Cell Biology

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    Tsonis, P. A., Del Rio-Tsonis, K., Rothrock, J., Dominguez, J., English, D., Glade, K., & Goetinck, P. F. (1991). Inhibitory effects of phospholipase D on chondrogenesis in vitro. Experimental Cell Research, 195(2), 551-555. https://doi.org/10.1016/0014-4827(91)90411-M