Inhibitory effects of phospholipase D on chondrogenesis in vitro

Panagiotis A. Tsonis, Katia Del Rio-Tsonis, James Rothrock, Jesus Dominguez, Denis English, Kimberly Glade, Paul F. Goetinck

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mesenchymal cells from the wing buds of stage 24 chick embryos undergo differentiation to cartilage when plated at high density. Treatment of these cultures with phospholipase D resulted in inhibition of chondrogenesis. Phospholipase D treatment (which produces phosphatidic acid from membrane phospholipids) was found to affect cell proliferation and to dramatically increase intracellular free calcium levels and inositol phosphate production. Intracellular free Ca2+, mobilized as a result of phosphatidylinositol phosphate hydrolysis, may therefore inhibit chondrogenesis in embryonic mesenchymal cells.

Original languageEnglish
Pages (from-to)551-555
Number of pages5
JournalExperimental Cell Research
Volume195
Issue number2
DOIs
StatePublished - 1991

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Chondrogenesis
Phospholipase D
Phosphatidylinositol Phosphates
Phosphatidic Acids
Inositol Phosphates
Chick Embryo
Cartilage
Phospholipids
Hydrolysis
Cell Proliferation
Calcium
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tsonis, P. A., Del Rio-Tsonis, K., Rothrock, J., Dominguez, J., English, D., Glade, K., & Goetinck, P. F. (1991). Inhibitory effects of phospholipase D on chondrogenesis in vitro. Experimental Cell Research, 195(2), 551-555. https://doi.org/10.1016/0014-4827(91)90411-M

Inhibitory effects of phospholipase D on chondrogenesis in vitro. / Tsonis, Panagiotis A.; Del Rio-Tsonis, Katia; Rothrock, James; Dominguez, Jesus; English, Denis; Glade, Kimberly; Goetinck, Paul F.

In: Experimental Cell Research, Vol. 195, No. 2, 1991, p. 551-555.

Research output: Contribution to journalArticle

Tsonis, PA, Del Rio-Tsonis, K, Rothrock, J, Dominguez, J, English, D, Glade, K & Goetinck, PF 1991, 'Inhibitory effects of phospholipase D on chondrogenesis in vitro', Experimental Cell Research, vol. 195, no. 2, pp. 551-555. https://doi.org/10.1016/0014-4827(91)90411-M
Tsonis, Panagiotis A. ; Del Rio-Tsonis, Katia ; Rothrock, James ; Dominguez, Jesus ; English, Denis ; Glade, Kimberly ; Goetinck, Paul F. / Inhibitory effects of phospholipase D on chondrogenesis in vitro. In: Experimental Cell Research. 1991 ; Vol. 195, No. 2. pp. 551-555.
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