Initial results of endothelial cell seeding following argon laser carotid endarterectomy

Alan P. Sawchuk, Malcolm B. Herring, Michael C. Dalsing

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This study evaluates the initial results of endothelial cell (EC) seeding following argon laser carotid endarterectomy. Venous endothelial cells were harvested from 12 dogs and cultured. A laser endarterectomy was performed on both carotids of each dog. One side was seeded with endothelial cells. Six dogs had both carotids harvested 1 hour after restoring blood flow. The others were harvested in 24 hours. The percentage of lumen covered with EC was evaluated by scanning electron microscopy. At 1 hour, the seeded arteries demonstrated 35 ± 3 percent EC coverage, whereas the unseeded arteries had no EC coverage (P = 0.0002). At 24 hours, the seeded arteries had 58 ± 15 percent EC coverage, whereas the unseeded arteries had no coverage (P = 0.01). Significant gross thrombus developed only in unseeded arteries (P = 0.047), two of which were occluded at 24 hours. EC seeding is beneficial following argon laser carotid endarterectomy resulting in improved patency and less surface thrombogenicity.

Original languageEnglish (US)
Pages (from-to)569-575
Number of pages7
JournalLasers in Surgery and Medicine
Volume12
Issue number6
DOIs
StatePublished - 1992

Keywords

  • argon laser
  • carotid endarterectomy
  • endothelial cell seeding

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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